2012

  • Rodrigues AF, Amaral AI, Veríssimo V, Alves PM, Coroadinha AS. 2012. Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production, Biotechnol Bioeng, in press, http://dx.doi.org/10.1002/bit.24410

2011

  • Silva AC; Carrondo MJT and Alves PM. 2011. Strategies for improved stability of Peste des Petits Ruminants Vaccine, Vaccine 2011, 29, 4983– 4991. http://dx.doi.org/10.1016/j.vaccine.2011.04.102

  • Tostoes R, Leite SB, Serra M, Jensen J, Björquist P, Carrondo M, Brito  C, Alves PM. 2011. Human liver cell spheroids in extended perfusion bioreactor  culture for repeated dose drug testing", Hepatology, in press; http://dx.doi.org/10.1002/hep.24760

  • Serra M, Correia C, Malpique R, Brito C, Jensen J, Bjorquist P, Carrondo MJT, Alves PM. 2011. Microencapsulation technology: a powerful tool to integrate expansion and cryopreservation of pluripotent human embryonic stem cells. PloS One 6(8). http://dx.plos.org/10.1371/journal.pone.0023212

  • Real G, Monteiro F, Burger C, Alves PM. 2011. Improvement of lentiviral transfer vectors using cis-acting regulatory elements for increased gene expression. Appl Microbiol Biotechnol, 91(6):1581-91. http://dx.doi.org/10.1007/s00253-011-3392-2

  • Amaral IA, Teixeira AP, Håkonsen BI, Sonnewald U, Alves PM. 2011. A comprehensive metabolic profile of cultured astrocytes using Isotopic Transient Metabolic Flux Analysis and 13C-labelled glucose. Front. Neuroenerg 3:5. http://dx.doi.org/10.3389/fnene.2011.00005

  • Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJT, Coroadinha AS. 2011. Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: Tailoring vector composition, Biotechnology and Bioengineering. in press. http://dx.doi.org/10.1002/bit.23231

  • Teixeira AP, Dias JML, Carinhas N, Sousa M, Clemente JJ, Cunha AE, Alves PM, Carrondo MJT, Oliveira R. 2011. Cell functional enviromics: unravelling the function of environmental factors, BMC Systems Biology, 5:92. http://dx.doi.org/10.1186/1752-0509-5-92

  • Vicente T, Mota JPB, Peixoto C, Alves PM, Carrondo MJT, 2011. Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: current advances. Biotechnology Advances, 29 (6) 869-878. http://dx.org/10.1016/B978-0-08-0885004-9.00072-6

  • Roldão A, Silva AC, Mellado MCM, Alves PM, and Carrondo MJT. 2011 1.47 - Viruses and Virus-Like Particles in Biotechnology: Fundamentals and Applications. Murray Moo-Young (ed.), Comprehensive Biotechnology, Second Edition, 1; 625:649. http://dx.doi.org/10.1016/B978-0-08-088504-9.00072-6

  • Vieira HL; Alves PM; Vercelli A. 2011. Modulation of neuronal stem cell differentiation by hypoxia and reactive oxygen species; Elsevier, in press;
    http://dx.doi.org/10.1016/j.pneurobio.2011.01.007

  • Leite SB, Teixeira AP, Miranda JP, Tostoes RM, Clemente JJ, Sousa MF, Carrondo MJT, Alves PM. 2011. Merging bioreactor technology with 3D hepatocyte-fibroblast culturing approaches: improved in vitro models for Toxicological applications, Toxicology in Vitro, in press;
    http://dx.doi.org/10.1016/j.tiv.2011.02.002

  • Teixeira AP, Duarte TM, Oliveira R, Carrondo MJTC, Alves PM. 2011 High-throughput analysis of animal cell cultures using two dimensional fluorometry, J. Biotechnology, 151:255-260;
    http://dx.doi.org/10.1016/j.jbiotec.2010.11.015

  • Roldão A, Vicente T, Peixoto C, Carrondo MJT, Alves PM, 2011. Quality control for baculovirus based products, J. Invertebr Pathol, in press.

  • Lamego J, Coroadinha AS, Simplicio AL; 2011 Detection and Quantification of Carboxylesterase 2 Activity by Capillary Electrophoresis. Analytical Chemistry, in press.
    http://dx.doi.org/10.1021/ac102547c

  • Vicente T, Peixoto C, Mota JPB, Carrondo MJT, Alves PM; 2011. Optimizing Downstream Processing of Enveloped Viruses: Case Study Demonstrates Utility of Novel Strategies, Genetic Engineering & Biotechnology News: Bioprocess Tutorial January 15.

  • Vicente T, Fáber R, Alves PM, Carrondo MJT, Mota JPB; 2011. Impact of ligand density on the optimization of ion-exchange membrane chromatography for viral vector purification. Biotechnol Bioeng, in press.
    http://dx.doi.org/10.1002/bit.23058

  • Vicente T, Roldão A, Peixoto C, Carrondo MJT, Alves PM; 2011. Large-scale production and purification of VLP-based vaccines, J Invertebr Pathol, in press.

  • Lesch HP, Laitinen A, Peixoto C, Vicente T, Makkonen ME, Laitinen L, Pikkarainen JT, Samaranayake H, Alves PM, Carrondo MJT, Ylä-Herttuala S, Airenne KJ. 2011. Production and Purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Therapy, in press.
    http://dx.doi.org/10.1038/gt.2010.162

  • Amaral A I , Teixeira AP, Sonnewald U, Alves PM; 2011. Estimation of intracellular fluxes in cerebellar neurons after hypoglyce m ia : importance of the pyruvate recycling pathway and glutamine oxidation. Journal of Neuroscience Research, in press.

2010

  • Serra M, Brito C, Alves PM; 2010. Bioengineering strategies for stem cell expansion and differentiation, Canal Bioquímica, 7:30-37.

  • Silva AC, Peixoto C, Lucas T, Kuppers C, Cruz P, Alves PM, kochanek S.; 2010. Adenovirus Vector Production and Purification. Curr. Gene Ther., 1;10(6):437-55.

  • Coroadinha AS, Gama-Norton L, Amaral AI, Hauser H, Alves PM, Cruz P; 2010. Production of Retroviral Vectors. Curr. Gene Ther., 1;10(6):456-73.

  • Vieira HL, Pereira AC, Peixoto C, Moraes RH, Alves PM, Mendonça RZ; 2010. Improvement of recombinant protein production by an anti-apoptotic protein from hemolymph of Lonomia obliqua. Cytotechnology, 62:547-555.
    http://dx.doi.org/10.1007/s10616-010-9305-x

  • Bernal V, Monteiro F, Carinhas N, Ambrósio R, Alves PM; 2010. An integrated analysis of enzyme activities, cofactor pools and metabolic fluxes in baculovirus-infected Spodoptera frugiperda Sf9 cells. J Biotechnol. 150:332-342.
    http://dx.doi.org/10.1016/j.jbiotec.2010.09.958

  • Roldão A, Mellado MC, Castilho LR, Carrondo MJ, Alves PM; 2010. Virus-like particles in vaccine development. Expert Rev Vaccines. 9(10):1149-76.
    http://dx.doi.org/10.1586/erv.10.115

  • Teixeira AP, Duarte TM, Oliveira R, Carrondo MJTC, Alves PM. 2010 High-throughput analysis of animal cell cultures using two dimensional fluorometry, J. Biotechnology, in press
    http://dx.doi.org/10.1016/j.jbiotec.2010.11.015

  • Tostões R, Leite S, Sousa M, Wang D, Carrondo MJT, Alves PM; 2010. Perfusion of 3D encapsulated hepatocytes – A synergistic effect enhancing long-term functionality in bioreactors. Biotechnol Bioeng. 108(1):41-49.
    http://dx.doi.org/10.1002/bit.22920

  • Serra M, Brito C, Sousa MFQ, Jensen J, Tostões RM, Clemente JJ, Hyllner J, Strehl R, Carrondo MJT, Alves PM; 2010. Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control. J. Biotechnology, in press.
    http://dx.doi.org/10.1016/j.jbiotec.2010.06.015

  • Carinhas N, Bernal V, Monteiro F, Carrondo MJT, Oliveira R, Alves PM; 2010. Improving baculovirus production at high cell density through manipulation of energy metabolism, Metabolic Engineering, 12: 39-52.
    http://dx.doi.org/10.1016/j.ymben.2009.08.008

  • Vicente T, Mota JPB, Peixoto C, Alves PM, Carrondo MJT; 2010. Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance, Journal of Chromatography A, 1217:2032-2041
    http://dx.doi.org/10.1016/j.chroma.2010.01.068

  • Vicente T, Peixoto C, Alves PM, Carrondo MJT; 2010. Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development, Journal of Chromatography A, 1217:3754-3764.
    http://dx.doi.org/10.1016/j.chroma.2010.03.059

  • Vicente T, Mota JPB, Peixoto C, Alves PM, Carrondo MJT; 2010 Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance, Journal of Biotechnology, in press.
    http://dx.doi.org/10.1016/j.jbiotec.2010.05.005

  • Amaral AI, Teixeira AP, Martens S, Sousa MFQ, Bernal V, Alves PM; 2010. Metabolic alterations induced by ischemia in primary cultures of astrocytes: merging (13)C NMR spectroscopy and metabolic flux analysis. Journal of Neurochemistry, 113(3): 48-735.
    http://dx.doi.org/10.1111/j.1471-4159.2010.06672.x

  • Queiroga CS, Almeida AS, Martel C, Brenner C, Alves PM, Vieira HL; 2010. Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilisation and apoptosis. Journal of Biological Chemistry, in press.
    http://dx.doi.org/10.1074/jbc.M109.065052

  • Miranda JP, Rodrigues A, Tostões RM, Leite S, Zimmerman H, Carrondo MJ, Alves PM; 2010,. Extending Hepatocyte Functionality for Drug-Testing Applications Using High-Viscosity Alginate-Encapsulated Three-Dimensional Cultures in Bioreactors. Tissue Eng Part C Methods, in press.
    http://dx.doi.org/10.1089/ten.tec.2009.0784

  • Franco CF, Mellado MC, Alves PM, Coelho AV; 2010, Monitoring virus-like particle and viral protein production by intact cell MALDI-TOF mass spectrometry. Talanta, 80(4):1561-8.
    http://dx.doi.org/10.1016/j.talanta.2009.06.081

  • Malpique R, Osório LM, Ferreira DS, Ehrhart F, Brito C, Zimmermann H, Alves PM; 2010. Alginate Encapsulation as a Novel Strategy for the Cryopreservation of Neurospheres. Tissue Eng Part C Methods, in press.
    http://dx.doi.org/10.1089/ten.tec.2009.0660

  • Matias AA, Serra AT, Silva AC, Perdigão R, Ferreira TB, Marcelino I, Silva S, Coelho AV, Alves PM, Duarte CM; 2010. Portuguese winemaking residues as a potential source of natural anti-adenoviral agents.Int J Food Sci Nutr., in press.
    http://dx.doi.org/10.3109/09637480903430990

  • Adakal H, Stachurski F, Konkobo M, Zoungrana S, Meyer DF, Pinarello V, Aprelon R, Marcelino I, Alves PM, Martinez D, Lefrancois T, Vachiéry N., 2010; Efficiency of inactivated vaccines against heartwater in Burkina Faso: Impact of Ehrlichia ruminantium genetic diversity, 28: 4573-4580.
    http://dx.doi.org/10.1016/j.vaccine.2010.04.087

2009

  • Mellado MCM, Mena JA, Lopes A, Ramirez OT, Carrondo MJT, Palomares LA, Alves PM; 2009. Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles. Biotechnology & Bioengineering, 104(4):674-86.
    http://dx.doi.org/10.1002/bit.22430

  • Rodrigues AF, Carmo M, Alves PM, Coroadinha AS; 2009. Retroviral vector production under serum deprivation: the role of lipids, Biotechnology and Bioengineering, in press.
    http://dx.doi.org/10.1002/bit.22499

  • Serra M, Brito C, Costa E, Sousa MFQ, Alves PM; 2009. Integrating human stem cell expansion and neuronal differentiation in bioreactors, BMC Biotechnology, 8: 92.
    http://www.biomedcentral.com/1472-6750/9/82

  • Teixeira AP, Oliveira R, Alves P, Carrondo MJT, 2009; Advances in real-time monitoring and control of mammalian cell cultures: supporting the PAT initiative. Biotechnol Advances, 27: 726-732.
    http://dx.doi.org/10.1016/j.biotechadv.2009.05.003

  • António Roldão, Rui Oliveira, Manuel J. T. Carrondo and Paula M. Alves; 2009. Error Assessment in Recombinant Baculovirus Titration: Evaluation of Different Methods. Journal of Virological Methods, 159: 69-80.
    http://dx.doi.org/10.1016/j.jviromet.2009.03.007

  • Bernal V, Carinhas N, Yokomizo A, Carrondo MJT, Alves PM; 2009. Cell density effect in the Baculovirus-Insect Cells system: a quantitative analysis of energetic metabolism. Biotechnology & Bioengineering, 104(1):162-180.
    http://dx.doi.org/10.1002/bit.22364

  • Carinhas N, Bernal V, Yokomizo AY, Carrondo MJT, Oliveira R, Alves PM; 2009. Baculovirus production for gene therapy: the role of cell density, MOI and medium exchange, Applied Microbiology and Biotechnology, 81(6):1041-1049.
    http://dx.doi.org/10.1007/s00253-008-1727-4

  • Ferreira TB, Perdigão R, Silva AC, Zhang C, Aunins JG, Carrondo MJT, Alves PM; 2009. 293 Cell Cycle Synchronisation in Adenovirus Vector Production, Biotechnology Progress, 25(1): 235-43.
    http://dx.doi.org/10.1002/btpr.64

  • I. Güll, Paula M. Alves, F Gabor, M Wirth; 2009. Viability of Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature. Scientia Pharmaceutica, open access.
    http://dx.doi.org/10.3797/scipharm.0810-07

  • M. Carmo, A. Alves, AF Rodrigues, AS Coroadinha, M. J. T. Carrondo, P. M. Alves and P. E. Cruz, 2009 Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer. The Journal of Gene Medicine, 11:670-678.
    http://dx.doi.org/10.1002/jgm.1353

  • Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM, 2009. Cryopreservation of Adherent Cells: Strategies to Improve Cell Viability and Function after Thawing, Tissue Engineering Part C, 15: 1-14.
    http://de.doi.org/10.1089/ten.tec.2009.0784

  • Miranda JP, Leite SB, Muller-Vieira U, Rodrigues A, Carrondo MJT, Alves PM, 2009. Toward Extended Functional Hepatocyte In Vitro Culture, Tissue Engineering Part C, 15: 1-11.
    http://dx.doi.org/10.1089=ten.tec.2008.0352

  • Serra M, Brito C, Leite SB, Gorjup E, von Briesen H, Carrondo MJT, Alves PM, 2009. Stirred Bioreactors for the Expansion of Adult Pancreatic Stem Cells, Annals of Anatomy, 191, 104-115.
    http://dx.doi.org/10.1016/j.aanat.2008.09.005

  • Teixeira AP, Portugal CAM, Carinhas N, Dias JML, Crespo JP, Alves PM, Carrondo MJT, Oliveira R. 2009. In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures, Biotechnology and Bioengineering, 102(4), 1098-1106.
    http://dx.doi.org/10.1002/bit.22125

  • Vicente T, Peixoto C, Carrondo MJT, Alves PM, 2009. Purification of recombinant baculoviruses for gene therapy using membrane processes, Gene Therapy, 16:766-775.
    http://dx.doi.org/10.1038/gt.2009.33

  • Vieira HL, Cunha L, Goldmacher VS, Alves PM. 2009. The effect of the cell death suppressor vMIA on the production of a recombinant protein in the adenovirus-293 expression system, Protein Expr Purif.; 64(2): 179-84.
    http://dx.doi.org/10.1016/j.pep.2008.11.004

  • Vicente T, Peixoto C, Carrondo MJ, Alves PM., 2009, Virus production for clinical gene therapy, Methods Mol Biol.; 542:447-70.
    http://dx.doi.org/10.1007/978-1-59745-561-9_24

  • Carmo M, Dias JD, Panet A, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE., 2009, Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors., Hum Gene Ther. 1168-76.
    http://dx.doi.org/10.1089/hum.2009.068

  • Bannenberg, G. L. and Vieira, H. L.; 2009. Therapeutic applications of the gaseous mediators carbon monoxide and hydrogen sulfide. Expert Opin Ther Pat 19, 663-82.
    http://dx.doi.org/10.1111/j.1471-4159.2010.06636.x

2008

  • Amaral AI, Coroadinha AS, Merten O-W, Alves PM, 2008. Improving retroviral vectors production: role of carbon sources in lipid biosynthesis, Journal of Biotechnology, 138 (2008) 57-66.
    http://dx.doi.org/10.1016/j.jbiotec.2008.08.005

  • Carrondo, M.J.T; Merten, O.-W.; Haury, M., Alves, P.M. and Coroadinha, A.S. 2008. Impact of Retroviral Vector Components Stoichiometry on Packaging Cell Lines: effects on productivity and vector quality. Human Gene Therapy, 19:199-210.
    http://dx.doi.org/doi:10.1089/hum.2007.0101

  • Clemente JJ, Monteiro SMS, Carrondo MJT, Cunha AE. 2008. Predicting sporulation events in a bioreactor using an Electronic Nose, Biotechnology and Bioengineering, 101(3):545-52.
    http://dx.doi.org/10.1002/bit.21920

  • Gouveia RM, Gomes CM, Sousa M, Alves, PM, Costa J, 2008. Kinetic analysis of L1 Homophilic Interaction: Role of the First Four Ig Domains and Implications on L1-L1 Binding Mechanism, Journal of Biological Chemistry, 283(42):28038-47.
    http://dx.doi.org/10.1074/jbc.M804991200

  • M. Carmo, A. Panet, M. J. T. Carrondo, P. M. Alves, P. E. Cruz, 2008, From retroviral vector production to gene transfer: spontaneous inactivation is caused by loss of reverse transcription capacity. The Journal of Gene Medicine, 10:383-391.
    http://dx.doi.org/10.1002/jgm.1163

  • Maria Candida M. Mellado, Catarina Franco, Ana Coelho, Paula M. Alves and Ana Luísa Simplício, 2008, Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles. Journal of Chromatography A, 1192:166-172.
    http://dx.doi.org/10.1016/j.chroma.2008.03.035

  • Mellado MC, Peixoto C, Cruz PE, Carrondo MJT, Alves PM. 2008. Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly, J Chromatography B, 874: 89-94.
    http://dx.doi.org/10.1016/j.jchromb.2008.09.002

  • Peixoto C, Ferreira TB, Sousa MQ, Carrondo MJT, Alves PM, 2008. Towards Purification of adenovirus vectors based on membrane technology, Biotechnology Progress, 24:1290-1296. http://dx.doi.org/10.1002/btpr.25

  • Perovski Z, Norton de Matos MRP., Braga SS, Pereira CCL, Matos ML, Gonçalves IS, Pillinger M, Alves PM, Romão CC, 2008. Synthesis, characterization and antitumor activity of 1,2-disubstituted ferrocenes and cyclodextrin inclusion complexes, Journal of Organometallic Chemistry, 693:675-684.
    http://dx.doi.org/10.1016/j.jorganchem.2007.11.053

  • Rodrigues T, Alves A, Lopes A, Carrondo MJT, Alves PM, Cruz PE, 2008. Removal of envelope protein-free retroviral vectors by anion exchange chromatography to improve product quality, Journal of Separation Science, 31:3509-3518.
    http://dx.doi.org/10.1002/jssc.200800195

  • Roldão, A, Carrondo, MJT, Alves, PM, Oliveira, R, 2008. Stochastic simulation of protein expression in the Baculovirus/Insect cell system, Computers and Chemical Engineering, 32: 68-77.
    http://dx.doi.org/10.1016/j.compchemeng.2007.04.017

  • Silva AC, Delgado I, Sousa MQ, Carrondo MJT, Alves PM, 2008. Scalable culture systems using different cell lines for the Production of Peste des Petits Ruminants Vaccine, Vaccine, 26:3305-11.
    http://dx.doi.org/10.1016/j.vaccine.2008.03.077

  • Teixeira, A. P., Santos, S. S., Carinhas, N., Oliveira, R., Alves, P. M. 2008. Combining Metabolic Flux Analysis tools and 13C-NMR to estimate intracellular fluxes of cultured astrocytes. Neurochemistry International, 52: 478-486.
    http://dx.doi.org/10.1016/j.neuint.2007.08.007

  • Vicente T., Sousa M. Q., Peixoto C., Mota J. P., Alves P. M., and Carrondo M. J. T., 2008. Anion exchange membrane chromatography for purification of rotavirus-like particles, Journal of Membrane Science, 311: 270-283.
    http://dx.doi.org/10.1016/j.memsci.2007.12.021

  • Vieira HLA, Queiroga CSF, Alves PM, 2008. Preconditioning induced by Carbon Monoxide provides neuronal protection against Apoptosis, Journal of Neurochemistry, 107:375-384.
    http://dx.doi.org/10.1111/j.1471-4159.2008.05610.x

2007

  • A.P. Teixeira, N. Carinhas, J.M. Dias, P. Cruz, P.M. Alves, M.J.T. Carrondo, R. Oliveira, 2007. Hybrid semi-parametric mathematical systems: bridging the gap between biology and process engineering, J Biotechnol,132: 418-425.
    http://dx.doi.org/10.1016/j.jbiotec.2007.08.020

  • Bento, I., Peixoto, C., Zaitsev, V.N. and Lindley, P., 2007. Ceruloplasmin revisited: structural and functional roles of various metal cation-binding sites, Acta Cryst., 63(2): 240-248.
    http://dx.doi.org/10.1107/S090744490604947X

  • Faburay B, Geysen D, Ceesay A, Marcelino I, Alves PM, Taoufik A, Postigo M, Bell-Sakyi L, Jongejan F., 2007. Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines, Vaccine, 25(46):7939-47.
    http://dx.doi.org/10.1016/j.vaccine.2007.09.002

  • Ferreira TB, Carrondo MJT, Alves PM, 2007. Effect of ammonia production on intracellular pH consequent effect on adenovirus vector production, Journal of Biotechnology, 129 (3); 433-438.
    http://dx.doi.org/10.1016/j.jbiotec.2007.01.010

  • Gouveia, RM, Morais, VA, Peixoto, C, Sousa, M., Regalla, M., Alves, P. M. , Costa J., 2007. Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells. Protein Expression and Purification, 52: 182-193.
    http://dx.doi.org/10.1002/bit.21382

  • Malpique, R, Katsen-Globa, A, Carrondo, MJT, Zimmermann, H, Alves, PM, 2007. Cryopreservation in micro-volumes: impact upon Caco-2 Colon Adenocarcinoma cells proliferation and differentiation, Biotechnology & Bioengineering 98: 155-66.
    http://dx.doi.org/10.1002/bit.21382

  • Marcelino I, Vachiéry N, Amaral AI, Roldão A, Lefrançois T, Carrondo MJ, Alves PM, Martinez D.. 2007. Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater, Vaccine, 25(26):4903-13.
    http://dx.doi.org/10.1016/j.vaccine.2007.04.055

  • Peixoto, C, Marcelino, I., Amaral, AI, Carrondo, MJT, Alves, PM, 2007. Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against Heartwater, Process Biochemistry, 42(7): 1084-1089.
    http://dx.doi.org/10.1016/j.procbio.2007.04.012

  • Peixoto, C, Sousa, MFQ, Silva, AC, Carrondo, MJT, Alves, PM, 2007. Downstream Processing of Triple-layered Rotavirus Like Particles, Journal of Biotechnology, 127, 452-461
    http://dx.doi.org/10.1016/j.jbiotec.2006.08.002

  • Rodrigues, T, Carrondo MJT, Alves, PM, Cruz, PE, 2007. Purification of retroviral vectors for clinical application: biological implication and technological challenges, Journal of Biotechnology, 127, 520-541.
    http://dx.doi.org/10.1016/j.jbiotec.2006.07.028

  • Rodrigues, T, Carvalho, A, Carmo, M., Carrondo MJT, Alves, PM, Cruz, 2007. Scaleable purification process for gene therapy retroviral vectors. Journal of Gene Medicine, 9(4): 233-243.
    http://dx.doi.org/10.1002/jgm.1021

  • Roldão, A, Vieira, HLA, Charpilienne, A, Poncet, D, Roy, P, Carrondo, MJT, Alves, PM, Oliveira, R, 2007. Modeling Rotavirus-Like Particles production: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production. Journal of Biotechnology, 128(4): 875-894.
    http://dx.doi.org/10.1016/j.jbiotec.2007.01.003

  • Sá Santos, S, Leite, SM, Sonnewald, U, Carrondo, MJT and Alves, PM, 2007. Stirrer vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics, Journal of Neuroscience Research, 85(15): 3386-3397.
    http://dx.doi.org10.1002/jnr.21409

  • Serra, M., Leite, S., Brito, C., Costa, J., Carrondo, M. J. T., Alves, P. M., 2007. A novel culture strategy for human stem cell proliferation and neuronal differentiation. Journal of Neuroscience Research, 85(16): 3557-3566.
    http://dx.doi.org/10.1002/jnr.21451

  • Teixeira, A, Alves, C., Alves, PM, Carrondo MJT, Oliveira R, 2007. Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control, BMC Bioinformatics, 8(1): 30.
    http://dx.doi.org/10.1186/1471-2105-8-30

  • Wirth, D., Gama-Norton, L., Riemer, P., Sandhu, U., Schucht, R. and Hauser, H., 2007. Road to precision: recombinase-based targeting technologies for genome engineering Current Opinion in Biotechnology, 18:411–419.
    http://dx.doi.org/10.1016/j.copbio.2007.07.013

2006

  • Antonis AF, Bruschke CJ, Rueda P, Maranga L, Casal JI, Vela C, Hilgers LA, Belt PB, Weerdmeester K, Carrondo MJ, Langeveld JP., 2006. A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure. Vaccine, 24(26): 5481-90.
    http://dx.doi.org/10.1016/j.vaccine.2006.03.089

  • Brennan, L., Alves, P.M., Hewage, C., Malthouse, J.G. and McBean, G.J., 2006. The gliotoxin L-serine-O-suphate impacts on cellular metabolism in cultured astrocytes, Neurochemistry International, 48: 739-45.
    http://dx.doi.org/10.1016/j.neuint.2005.12.004

  • Carmo, M., Faria, T.Q., Falk, H., Coroadinha, A.S., Teixeira, M., Merten, O.-W., Geny-Fiamma, C., Alves, P.M., Danos, O., Panet, A., Carrondo, M.J.T. and Cruz, P.E., 2006. Relationship between retroviral vector membrane and vector stability. J. Gen. Virol., 87(Pt 5): 1349-56.
    http://dx.doi.org/10.1099/vir.0.81302-0

  • Coroadinha, A.S., Alves, PM., Sá Santos, Sónia, Cruz, P.E., Merten, O.-W., Carrondo, M.J.T., 2006. Retrovirus producer cell line metabolism: implications on viral productivity. Appl Microbiol Biotechnol. 72(6): 1125-1135.
    http://dx.doi.org/10.1007/s00253-006-0401-y

  • Coroadinha, A.S., Ribeiro, J., Roldão, A., Cruz, P.E., Alves, PM., Merten, O.-W., Carrondo, M.J.T., 2006. Effect of medium sugar source on the production of retroviral vectors for gene therapy. Biotechnology & Bioengineering, 94(1): 24-36.
    http://dx.doi.org/10.1002/bit.20778

  • Coroadinha, A.S., Schucht, R., Gama-Norton, L., Wirth, D., Hauser, H., Carrondo, M.J.T., 2006. The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchange. Journal of Biotechnology, 124(2): 457-68.
    http://dx.doi.org/10.1016/j.jbiotec.2006.01.037

  • Coroadinha, A.S., Silva, A.C., Pires, E., Coelho, A., Alves, P.M., Carrondo, M.J.T., 2006. Effect of osmotic Pressure on the Production of Retroviral Vectors: Enhancement in Vector Stability. medium sugar source on the production of retroviral vectors for gene therapy Biotechnology & Bioengineering, 94(2): 322-9.
    http://dx.doi.org/10.1002/bit.20847

  • Cruz PE, Silva AC, Roldao A, Carmo M, Carrondo MJ, Alves PM., 2006. Screening of novel excipients for improving the stability of retroviral and adenoviral vectors. Biotechnol Prog, 22(2): 568-76.
    http://dx.doi.org/10.1021/bp050294y

  • Lameiro, MH, Lopes, A., Martins, L.O., Alves P.M. and Melo, E., 2006. Incorporation of a Model Protein into Chitosan-Bile salt Microparticles, International Journal of Pharmaceutics, 312: 119-30.
    http://dx.doi.org/10.1016/j.ijpharm.2006.01.006

  • Lameiro, MH, Malpique, R, Silva, AC, Alves P.M. and Melo, E., 2006. Encapsulation of adenoviral vectors into chitosan-bile salt microparticles for mucosal vaccination, Journal of Biotechnology, 126, 152-162.
    http://dx.doi.org/10.1016/j.jbiotec.2006.04.030

  • Marcelino I, Sousa MFQ, Veríssimo C, Cunha, A., Carrondo MJT, Alves PM., 2006. Process development for the mass production of Ehrlichia ruminantium. Vaccine, 24(10): 1716-25.
    http://dx.doi.org/10.1016/j.vaccine.2005.08.109

  • Peixoto C., Ferreira TB, Carrondo M.J.T., Cruz PE, Alves P.M., 2006. Purification of adenoviral vectors using expanded bed chromatography. Journal of Virological Methods, 132: 121-6.
    http://dx.doi.org/10.1016/j.jviromet.2005.10.002

  • Rodrigues T, Carvalho A, Roldao A, Carrondo MJ, Alves PM, Cruz PE., 2006. Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors. J Chromatogr B Analyt Technol Biomed Life Sci., 837(1-2): 59-68.
    http://dx.doi.org/10.1016/j.jchromb.2006.03.061

  • Roldão, A, Vieira HLA, Alves, PM, Oliveira, R, Carrondo, MJT, 2006. Intracellular dynamics in Rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies. Process Biochemistry, 41, 2188-2199.
    http://doi:10.1016/j.procbio.2006.06.019

  • Sá Santos, Sónia, Gibson, Gary E., Cooper, Arthur J.L., Denton, Travis T., Thompson, Charles M., Bunik, Victoria I., Alves, Paula M. and Sonnewald, Ursula, 2006. Inhibitors of the a-Ketoglutarate Dehydrogenase Complex Alter [1-13C]Glucose and [U-13C]Glutamate Metabolism in Cerebellar Granule Neurons, Journal of Neuroscience Research, 83:450-8.
    http://dx.doi.org/10.1002/jnr.20749

  • Schucht, R., Coroadinha, A.S., Zanta-Boussif, M.A., Verhoeyen, E., Carrondo, M.J.T., Hauser, H. and Wirth, D., 2006. A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors. Mol. Ther., 14(2): 285-92.
    http://dx.doi.org/10.1016/j.ymthe.2005.12.003

  • Teixeira AP, Clemente JJ, Cunha AE, Carrondo MJ, Oliveira R., 2006. Bioprocess iterative batch-to-batch optimization based on hybrid parametric/nonparametric models. Biotechnol Prog, 22(1): 247-58.
    http://dx.doi.org/10.1021/bp0502328

  • Vieira H.L.A., Pereira A.C.P., Carrondo M.J.T. and Alves P.M., 2006. Catalase effect on apoptosis for the improvement of recombinant protein production in baculovirus-insect cell system, at Bioprocess Biosystems Eng., 29 (5-6):409-14.
    http://dx.doi.org/10.1007/s00449-006-0093-9

  • Zhang, C., Ferreira TB, Cruz, P.E., Alves, P.M., Haury, M. and Carrondo, M.J.T., 2006. The importance of 293 cell cycle phase on adenovirus vector production, Enzyme Microbiology and Technology, 39: 1328-32.
    http://dx.doi.org/10.1016/j.enzmictec.2006.03.021

2005

  • Ferreira TB, Ferreira AL, Carrondo MJT, Alves PM 2005. Effect of refeed strategies and non-ammoniagenic medium on adenovirus production at high cell densities, Journal of Biotechnology,119: 272-280.
    http://dx.doi.org/10.1016/j.jbiotec.2005.03.009

  • Ferreira TB, Ferreira AL, Carrondo MJT, Alves PM, 2005. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnology Letters, 27(22): 1809-13.
    http://dx.doi.org/10.1007/s10529-005-3558-z

  • Ferreira, T.B., Alves, P.M., Aunins, J.G. Carrondo, M.J.T., 2005. Use of adenoviral vectors as veterinary vaccines, Gene Therapy, 12(S1): 73-83.
    http://dx.doi.org/10.1038/sj.gt.3302618

  • Fonseca L.L., Monteiro MAR, Alves P.M., Carrondo M.J.T., Santos H 2005. Cultures of rat astrocytes challenged with a steady supply of glutamate: a new model to study flux distribution in the glutamate-glutamine cycle, Glia, 51: 286-296.
    http://dx.doi.org/10.1002/glia.20209

  • Marcelino I, Veríssimo C, Sousa MFQ, Carrondo MJT, Alves PM, 2005. Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures, Veterinary Microbiology, 110: 87-96.
    http://dx.doi.org/10.1016/j.vetmic.2005.07.012

  • Peixoto, C.C., Marcelino, I., Sousa, M.Q., Vachiéry, N., Bensaid, A., Martinez D, Carrondo M.J.T., Alves, P.M. 2005. Quantification of Ehrlichia ruminantium By Real Time PCR, Veterinary Microbiology, 107: 273-8.
    http://dx.doi.org/10.1016/j.vetmic.2005.02.001

  • Sá Santos, Sónia, Fonseca, Luís L., Monteiro, Miguel A. R., Carrondo, M. J. T., Alves, P. M., 2005. Culturing primary brain astrocytes under fully controlled environment in a novel bioreactor, Journal of Neuroscience Research, 79:26-32.
    http://dx.doi.org/10.1002/jnr.20279

  • Souza A.P.B., Peixoto C.C., Maranga L., Carvalhal A.V., Moraes R.H.P., Mendonça R.Z.M., Pereira C.A., Carrondo M.J.T., Mendonça R.Z. 2005. "Purification and Characterization of an Anti-Apoptotic Protein Isolated from Lonomia obliqua Hemolymph" Biotechnology Progress. 21. 99-105.
    http://dx.doi.org/10.1021/bp049831p

  • Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJT, Oliveira R 2005. Modeling and optimisation of a recombinant BHK-21 cultivation process using hybrid grey-box systems, Journal of Biotechnology, 118: 290-303.
    http://dx.doi.org/10.1016/j.jbiotec.2005.04.024

  • Vieira HLA, Estevão C, Roldão, A, Peixoto, C, Sousa, M, Cruz, PE, Carrondo MJT, Alves, PM. 2005. Triple layered rotavirus VLP assembly: kinetics of vector replication, mRNA stability and recombinant protein production, Journal of Biotechnology, 120 (1): 72-82.
    http://dx.doi.org/10.1016/j.jbiotec.2005.03.026

  • Monteiro SM, Clemente JJ, Henriques AO, Gomes RJ, Carrondo MJ, Cunha AE, 2005, A procedure for high-yield spore production by Bacillus subtilis., Biotechnol Prog.;21(4):1026-31.
    http://dx.doi.org/10.1021/bp050062z

  • Kreij K, Mandenius CF, Clemente JJ, Cunha AE, Monteiro SM, Carrondo MJ, Hesse F, de Los Molinas MM, Wagner R, Merten OW, Gény-Fiamma C, Leger W, Wiesinger-Mayr H, Müller D, Katinger H, Mårtensson P, Bachinger T, Mitrovics J., 2005; On-line detection of microbial contaminations in animal cell reactor cultures using an electronic nose device. Cytotechnology.; 48(1-3):41-58.
    http://dx.doi.org/10.1007/s10616-005-3587-4

  • Oliveira R, Clemente JJ, Cunha AE, Carrondo MJ., 2005; Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation. J Biotechnol; 116(1):35-50.
    http://dx.doi.org/10.1016/j.jbiotec.2004.09.016

2004

  • Carmo, M., Peixoto, C. C., Coroadinha, A. S., Alves, P. M., Cruz, P. E., and Carrondo, M. J. T.. 2004. Quantitation of MLV-based retroviral vectors using real-time RT-PCR. J Virol Methods 119: 115-119.
    http://dx.doi.org/10.1016/j.jviromet.2004.03.007

  • Maranga, L., Cunha, A., Clemente, J., Cruz, P. E., Carrondo, M. J. T.. 2004. Scale-up of virus-like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity. J Biotechnol 107: 55-64.
    http://dx.doi.org/10.1016/j.jbiotec.2003.09.012

  • Carrondo MJ, Aunins JG., 2004; Biochemical engineering. Curr Opin Biotechnol; 15(5):441-3.
    http://dx.doi.org/10.1016/j.copbio.2004.08.014

  • Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJ., 2004; Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng; 86(4):458-67.
    http://dx.doi.org/10.1002/bit.20051

2003

  • Carvalhal, A. V., Marcelino, I., and Carrondo, M. J. T.. 2003a. Metabolic changes during cell growth inhibition by p27 overexpression. Appl Microbiol Biotechnol 63: 164-173.
    http://dx.doi.org/10.1007/s00253-003-1385-5

  • Carvalhal, A. V., Sá Santos, Sónia, Calado, J., Haury, M., and Carrondo, M. J. T.. 2003b. Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins. Biotechnol Prog 19: 69-83.
    http://dx.doi.org/10.1021/bp0255917

  • Maranga, L., Brazão, T. F. , and Carrondo, M. J. T.. 2003. Virus like particles production at low multiplicities of infection with the baculovirus insect cell system. Biotechnol Bioeng 84: 245-253.
    http://dx.doi.org/10.1002/bit.10773

  • Maranga, L., Mendonca, R. Z., Bengala, A., Peixoto, C. C., Moraes, R. H., Pereira, C. A., and Carrondo, M. J. T.. 2003. Enhancement of sf-9 cell growth and longevity through supplementation of culture medium with hemolymph. Biotechnol Prog 19: 58-63.
    http://dx.doi.org/10.1021/bp025583q

  • Moreira, J. L., Miranda, P. M., Alves, P. M., Marcelino, I., and Carrondo, M. J. T.. 2003. Culture methods for mass production of ruminant endothelial cells. In: B. Saha (ed.) Fermentation biotechnology. Acs symposium series No. 862. p 124-141. Oxford.
    http://dx.doi.org/10.1021/bk-2003-0862.ch008

2002

  • Carvalhal, A., Coroadinha, A., Alves, P., Moreira, J., Hauser, H., and Carrondo, M. 2002. Metabolic changes during cell growth inhibition by the irf-1 system. Enzyme and Microbial Technology 30: 95-109.
    http://dx.doi.org/10.1016/S0141-0229(01)00460-4

  • Cruz, H.J., Conradt, H. S., Dunker, R., Peixoto, C. M., Cunha, A. E., Thomaz, M., Burger, C., Dias, E. M., Clemente, J. , Moreira, J. L., Reike, E., and M. J. Carrondo 2002a. Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by bhk cells. J Biotechnol 96: 169-183.
    http://dx.doi.org/10.1016/S0168-1656(02)00028-7

  • Cruz, P. E., L. Maranga, and M. J. Carrondo. 2002b. Integrated process optimization: Lessons from retrovirus and virus-like particle production. J Biotechnol 99: 199-214.
    http://dx.doi.org/10.1016/S0168-1656(02)00210-9

  • Maranga, L., A. S. Coroadinha, and M. J. Carrondo. 2002a. Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: Effects on baculovirus adsorption and infection kinetics. Biotechnol Prog 18: 855-861.
    http://dx.doi.org/0.1021/bp025514b

  • Maranga, L., P. E. Cruz, J. G. Aunins, and M. J. Carrondo. 2002b. Production of core and virus-like particles with baculovirus infected insect cells. Adv Biochem Eng Biotechnol 74: 183-206.
    http://dx.doi.org/10.1007/3-540-45736-4

  • Maranga, L., Rueda, P., Antonis, A. F., Vela, C., Langeveld, J. P., Casal, J. I., and Carrondo, M. J. 2002c. Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol 59: 45-50.
    http://dx.doi.org/10.1007/s00253-002-0976-x

  • Lopes Mde F, Leitão AL, Regalla M, Marques JJ, Carrondo MJ, Crespo MT.; 2002; Characterization of a highly thermostable extracellular lipase from Lactobacillus plantarum. Int J Food Microbiol; 76(1-2):107-15.
    http://dx.doi.org/10.1016/S0168-1605(02)00013-2

2001

  • Barbotin, J.-N., J.-C. Portais, P. M. Alves, and H. Santos, 261 pp. 54 Springer, Ringbuch. 2001. Nmr and immobilized cells. In: W. R (ed.) Immobilized cells - springer lab manual No. 261. p 54. Springer, Ringbuch.

  • Carvalhal, A. V., J. L. Moreira, and M. J. Carrondo. 2001. Strategies to modulate bhk cell proliferation by the regulation of irf-1 expression. J Biotechnol 92: 47-59.
    http://dx.doi.org/10.1016/S0168-1656(01)00365-0

  • Fonseca, L. L., P. M. Alves, M. J. Carrondo, and H. Santos. 2001. Effect of ethanol on the metabolism of primary astrocytes studied by (13)c- and (31)p-nmr spectroscopy. J Neurosci Res 66: 803-811.
    http://dx.doi.org/10.1002/jnr.10039

  • Merten, O. W., Cruz, P. E., Rochette, C., Geny-Fiamma, C., Bouquet, C., Goncalves, D., Danos, O., and Carrondo, M. J. 2001. Comparison of different bioreactor systems for the production of high titer retroviral vectors. Biotechnol Prog 17: 326-335.
    http://dx.doi.org/10.1021/bp000162z

  • Morais, V. A., Serpa, J., Palma, A. S., Costa, T., Maranga, L., Costa, J., Wu, N., and Ataai, M. M. 2001. Expression and characterization of recombinant human alpha-3/4-fucosyltransferase iii from spodoptera frugiperda (sf9) and trichoplusia ni (tn) cells using the baculovirus expression system. Production of viral vectors for gene therapy applications. Biochem J 353: 719-725.
    http://www.biochemj.org/bj/353/bj3530719.htm

  • Fonseca LL, Alves PM, Carrondo MJ, Santos H., 2001; Effect of ethanol on the metabolism of primary astrocytes studied by (13)C- and (31)P-NMR spectroscopy.; J Neurosci Res; 66(5):803-11.
    http://dx.doi.org/10.1002/jnr.10039

2000

  • Alves, P. M., Fonseca, L. L., Peixoto, C. C., Almeida, A. C., Carrondo, M. J., and Santos, H. 2000a. Nmr studies on energy metabolism of immobilized primary neurons and astrocytes during hypoxia, ischemia and hypoglycemia. NMR Biomed 13: 438-448.
    http://dx.doi.org/10.1002/nbm.665

  • Alves, P. M., Nunes, R., Zhang, C., Maycock, C. D., Sonnewald, U., Carrondo, M. J., and Santos, H. 2000b. Metabolism of 3-(13)c-malate in primary cultures of mouse astrocytes. Dev Neurosci 22: 456-462.
    http://dx.doi.org/10.1159/000017475

  • Araujo, C., S. Marques, M. Carrondo, and L. Gonçalves. 2000. In vitro response of brwon bullhead catfish (bb) and rainbow trout (rtg-2) cell lines to benzo[a]pyrene. The Science of the Total Environment 247: 127-135.
    http://dx.doi.org/10.1016/S0048-9697(99)00484-2

  • Carvalhal, A. V., Moreira, J. L., Cruz, H., Mueller, P., Hauser, H., and Carrondo, M. J. T. 2000. Manipulation of culture conditions for bhk cell growth inhibition by irf-1 activation. Cytechnology 32: 135-145.
    http://dx.doi.org/10.1023/A:1008139304964

  • Cruz, H., E. Dias, C. Peixoto, J. Moreira, and M. Carrondo. 2000a. Product quality of a recombinant fusion protein expressed in immobilised baby hamster cells grown in protein-free medium. Biotechnology Letters 22: 677-682.
    http://dx.doi.org/10.1023/A:1005631602517

  • Cruz, H. J., C. Freitas, P. Alves, J. L. Moreira, and M. J. Carrondo. 2000b. Effects of ammonia and lactate on growth, metabolism, and productivity of bhk cells. Enzyme and Microbial Technology 27: 45-52.
    http://dx.doi.org/10.1016/S0141-0229(00)00151-4

  • Cruz, H. J., J. L. Moreira, and M. J. Carrondo. 2000c. Metabolically optimised bhk cell fed-batch cultures. Journal of Biotechnology 80: 109-118.
    http://dx.doi.org/10.1016/S0168-1656(00)00254-6

  • Cruz, H. J., Peixoto, C. M., Nimtz, M., Alves, P. M., Dias, E. M., Moreira, J. L., and Carrondo, M. J. 2000d. Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in bhk cells. Biotechnology and Bioengineering 69: 129-139.
    http://dx.doi.org/10.1002/(SICI)1097-0290(20000720)69:2<129::AID-BIT2>3.0.CO;2-5

  • Cruz, P. E., J. S. Almeida, P. N. Murphy, J. L. Moreira, and M. J. Carrondo. 2000e. Modeling retrovirus production for gene therapy. 1. Determination of optimal bioreaction mode and harvest strategy. Biotechnol Prog 16: 213-221.
    http://dx.doi.org/10.1021/bp9901466

  • Cruz, P. E., D. Goncalves, J. Almeida, J. L. Moreira, and M. J. Carrondo. 2000f. Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing. Biotechnol Prog 16: 350-357.
    http://dx.doi.org/10.1021/bp000017l

  • Cruz, P. E., Peixoto, C. C., Devos, K., Moreira, J. L., Saman, E., and Carrondo, M. J. 2000g. Characterization and downstream processing of hiv-1 core and virus-like-particles produced in serum free medium. Enzyme and Microbial Technology 26: 61-70.
    http://dx.doi.org/10.1016/S0141-0229(99)00128-3

1999

  • Burger, C., Carrondo, M. J., Cruz, H., Cuffe, M., Dias, E., Griffiths, J. B., Hayes, K., Hauser, H., Looby, D., Mielke, C., Moreira, J. L., Rieke, E., Savage, A. V., Stacey, G. N., and Welge, T. 1999. An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in bhk cells. Appl Microbiol Biotechnol 52: 345-353.
    http://dx.doi.org/10.1007/s002530051530

  • Cruz, H. J., A. S. Ferreira, C. M. Freitas, J. L. Moreira, and M. J. Carrondo. 1999a. Metabolic responses to different glucose and glutamine levels in baby hamster kidney cell culture. Applied Microbiology and Biotechnology 51: 579-585.
    http://dx.doi.org/10.1007/s002530051435

  • Cruz, H. J., J. L. Moreira, and M. J. Carrondo. 1999b. Metabolic shifts by nutrient manipulation in continuous cultures of bhk cells. Biotechnology and Bioengineering 66: 104-113.
    http://dx.doi.org/10.1002/(SICI)1097-0290(1999)66:2%3C104::AID-BIT3%3E3.3.CO;2-R

  • Cruz, P. E., Martins, P. C., Alves, P. M., Peixoto, C. C., Santos, H., Moreira, J. L., and Carrondo, M. J. 1999c. Proteolytic activity in infected and non infected insect cells: Degradation of hiv-1 pr55gag particles. Biotechnol Bioeng 65: 133-143.
    http://dx.doi.org/10.1002/(SICI)1097-0290(19991020)65:2%3C133::AID-BIT2%3E3.0.CO;2-X

  • Mueller, P., Carvalhal, A. V., Moreira, J. L., Geserick, C., Schroeder, K., Carrondo, M. J. T., and Hauser, H. 1999. Development of an irf-1 based proliferation control system. In: M. Al-Rubeai (ed.) Cell engineering. p 220-238. Kluwer Academic Publishers.

  • Silva Lopes Mde F, Leitão AL, Marques JJ, Carrondo MJ, Crespo MT., 1999; Processing of extracellular lipase of Lactobacillus plantarum: involvement of a metalloprotease. FEMS Microbiol Lett.; 176(2):483-7.
    http://dx.doi.org/10.1016/S0378-1097(99)00276-1

  • Lopes Mde F, Cunha AE, Clemente JJ, Carrondo MJ, Crespo MT., 1999; Influence of environmental factors on lipase production by Lactobacillus plantarum.; Appl Microbiol Biotechnol; 51(2):249-54.
    http://dx.doi.org/10.1007/s002530051389

1998

1997

  • Alves, P. M., M. J. T. Carrondo, and H. Santos. 1997. Immobilization of primary brain cells in porous microcarriers for on-line nmr spectroscopy. In: K. A. Pub (ed.) Animal cell technology. p 91-98.

  • Cruz, H. J., E. M. Dias, J. L. Moreira, and M. J. Carrondo. 1997a. Cell-dislodging methods under serum-free conditions. Applied Microbiology and Biotechnology 47: 482-488.
    http://dx.doi.org/10.1007/s002530050960

  • Cruz, P., J. Moreira, and M. Carrondo. 1997b. Insect cell growth evaluation during serum-free adaptation in stirred suspension cultures. Biotechnology Techniques 11: 117-120.
    http://dx.doi