In this work, combined
strategies for the cryopreservation of adherent cells were investigated based
on cell entrapment in clinical grade, ultra-high viscosity alginate using two
cell lines, neuroblastoma N2a and colon adenocarcinoma Caco-2, as model systems.
As the cryopreservation medium, serum-free CryoStorTM(Biolife
Solutions®) solution was compared with culture medium, both
containing 10% Me2SO. Our results show that cell entrapment beneath
an alginate layer significantly improves cell recovery post-thawing and that
the combination of gel entrapment and CryoStorTMmedium results in
cell specific responses to cryopreservation that lead to optimized protocols,
which is of great importance on the establishment of cryobanks of cell
monolayers to be available for cell.therapy and pharmacological applications.
Também a revistaTissue Engineering, onde o artigo está publicado, escolheu uma fotografia deste trabalho para a capa do seu Número 3 do Volume 15 da sua edição.
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Doutora Paula Alves fala sobre o desenvolvimento de vacinas de nova geração para o Portal Universia.pt
