Research on functional personalized cells published on Nature Communications


Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds.  However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, researchers expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.


Expansion of functional personalized cells with specific transgene combinations.

Christoph Lipps1,19, Franziska Klein2, Tom Wahlicht1, Virginia Seiffert2, Milada Butueva1, Jeannette Zauers3,Theresa Truschel3, Martin Luckner3, Mario Köster2, Roderick MacLeod4, Jörn Pezoldt5, Jochen Hühn5,Qinggong Yuan6,7, Peter Paul Müller2, Henning Kempf8, Robert Zweigerdt8, Oliver Dittrich-Breiholz9,Thomas Pufe10, Rainer Beckmann10, Wolf Drescher11,12, Jose Riancho13, Carolina Sañudo13, Thomas Korff14,Bertram Opalka15, Vera Rebmann16, Joachim R. Göthert15, Paula M. Alves17, Michael Ott6,7, Roland Schucht3,Hansjörg Hauser2, Dagmar Wirth1,18 & Tobias May3

1Model Systems for Infection and Immunity, HZI–Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124 Braunschweig, Germany. 2Department ofGene Regulation and Differentiation, HZI–Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124 Braunschweig, Germany. 3InSCREENeX GmbH,Inhoffenstr. 7, 38124 Braunschweig, Germany. 4Leibniz Institute DSMZ–German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7, 38124Braunschweig, Germany. 5Experimental Immunology, HZI–Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124 Braunschweig, Germany. 6Department of Gastroenterology, Hepatology, Endocrinology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. 7TranslationalResearch Group Cell and Gene Therapy, Twincore - Centre for Experimental and Clinical Infection Research GmbH, Feodor-Lynen-Str. 7, 30625 Hannover,Germany. 8Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, MHH, Carl-Neuberg-Str. 1, 30625Hannover, Germany. 9Research Core Unit Genomics, Medical School Hannover, 30625 Hannover, Germany. 10Department of Anatomy and Cell Biology,RWTH Aachen University, 52074 Aachen, Germany. 11Department of Orthopaedics, Aachen University Hospital, RWTH Aachen University, Aachen 52074,Germany. 12Department of Orthopedic Surgery of the Lower Limb and Arthroplasty, Rummelsberg Hospital, Schwarzenbruck 90592, Germany. 13Department of Internal Medicine, Hospital U.M. Valdecilla, University of Cantabria, IDIVAL, 39008 Santander, Spain. 14Institute of Physiology and Pathophysiology, RG Blood Vessel Remodeling, University Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany. 15Department of Hematology,West German Cancer Center (WTZ), University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany. 16Institute for Transfusion Medicine, UniversityHospital Essen, Virchowstr. 179, 45147 Essen, Germany. 17Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras 2781-901,Portugal. 18Experimental Hematology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. 19Present address: Experimental Cardiology, Justus-Liebig University Giessen, Aulweg 129, 35392 Giessen, Germany.