Selected Publications


Alves, M., Lamego, J., Bandeiras, T., Castro, R., Tomas, H., Coroadinha, A.S., Costa, J., and Simplício, A.L.
Human Carboxylesterase 2: studies on the role of glycosylation for enzymatic activity
Biochemistry and Biophysics Reports 5, 105-10. More info

Carinhas N, Pais DAM, Koshkin A, Fernandes P, Coroadinha A, Carrondo MJT, Alves PM, Teixeira AP
Metabolic flux profilling of MDCK cells during growth and canine adenovirus vector production
Scientific Reports 6: 23529 More info

Correia C, Koshkin A, Carido M, Espinha N, Šarić T, Lima PA, Serra M, Alves PM
Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing
Stem Cells Transl Med More info

Cunha B, Silva RJ, Aguiar T, Serra M, Daicic J, Maloisel JL, Clachan J, Åkerblom A, Carrondo MJ, Peixoto C, Alves PM.
Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography
J Chromatogr A. 2016 Jan 15;1429:292-303. More info

Estrada MF, Rebelo SP, Davies EJ, Pinto MT, Pereira H, Santo VE, Matthew J. Smalley, Barry ST, Gualda EJ, Alves PM, Anderson E, Brito C
Modelling the tumour microenvironment in long-term microencapsulated 3D cocultures recapitulates phenotypic features of disease progression
Biomaterials  More info

Fernandes CS, Castro R, Coroadinha AS, Roque AC
Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope
J Chromatogr A. pii: S0021-9673(16)30125-X More info

Gomes-Alves P, Serra M, Brito C, Ricardo CP, Cunha R, Sousa MF, Sanchez B, Bernad A, Carrondo MJT, Rodriguez-Borlado L, Alves PM
In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products
Translational Research More info

Patrone M, Coroadinha AS, Teixeira AP, Alves PM.
Palmitoylation strengthens cholesterol-dependent multimerization and fusion activity of human cytomegalovirus gB
Journal of Biochemical Chemistry More info

Santo VE, Estrada MF, Rebelo SP, Abreu S, Silva I, Pinto C, Veloso SC, Serra AT, Boghaert E, Alves PM, Brito, C
Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models
J Biotechnol. 221 118-29 More info

Simao D, Pinto C, Fernandes P, Piersanti S, Saggio I, Collinson LM, Schiavo G, Kremer EJ, Alves PM & Brito C
Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model
Gene Therapy, 23(1):86-94 More info

Soares HR, Castro R, Tomas HA, Rodrigues AF, Gomes-Alves P, Bellier B, Klatzmann D, Carrondo MJ, Alves PM, Coroadinha AS
Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity
Vaccine More info


Castro R, Fernandes P, Laske T, Sousa MFQAppl Microbiol Biotechnol , Genzel Y, Scharfenberg K, Alves PM, Coroadinha AS
Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells
Appl Microbiol Biotechnol (in press) More info

Cunha B, Peixoto C, Silva MM, Carrondo MJT, Serra M, Alves PM
Filtration methodologies for the clarification and concentration of human mesenchymal stem cells
J Memb Sci, Volume 478, 117-129  More info

Cunha, B., Aguiar, T., Silva, M.M., Silva, R.J., Sousa, M.F., Pineda, E., Peixoto, C., Carrondo, M.J., Serra, M., and Alves, P.M.
Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells.
J Biotechnol 213, 97-108. More info

Fernandes P, Almeida AI, Kremer EJ, Alves PM & Coroadinha AS
Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation
Scientific Reports 5: 9135 More info

Fernandes P, Simao D, Guerreiro MR, Kremer EJ, Coroadinha AS & Alves PM
Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors
Gene Therapy 22, 40-49 More info

Gomes J, Gomes-Alves P, Carvalho SB, Peixoto C, Alves PM, Altevogt P, Costa J
Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures
Biomolecules 2015, 5(3), 1741-1761. More info

Gomes-Alves, P., Serra, M., Brito, C., R-Borlado L, Lopez JA, Vazquez J, Carrondo MJT, Bernad A & Alves PM
Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome
Proteomics 15, 1332-7 More info

Gualda, E.J., Pereira, H., Vale, T., Estrada, M.F., Brito, C., and Moreno, N.
SPIM-fluid: open source light-sheet based platform for high-throughput imaging
Biomedical Optics Express 6, 4447-4456. More info

Kleiderman S, Sá JV, Teixeira AP, Brito C, Gutbier S, Evje LG, Hadera MG, Glaab E, Henry M, Sachinidis A, Alves PM, Sonnewald U, Leist M.
Functional and phenotypic differences of pure populations of stem cell-derived astrocytes and neuronal precursor cells
Glia. 2015 Dec 22. More info

Materne EM, Ramme AP, Terrasso AP, Serra M, Alves PM, Brito C, Sakharov DA, Tonevitsky AG, Lauster R, Marx U
A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing.
J Biotechnol 205, 36-46. More info

Nestola P, Peixoto C, Silva RJS, Alves PM, Mota JPB, Carrondo MJT
Improved virus purification processes for vaccines and gene therapy
Biotechnology and Bioengineering, 112(5):843-57 More info

Nestola P, Peixoto C, Villain L, Alves PM, Carrondo MJT, Mota JPB.
Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles
Journal of Chromatography A 1426, 91-101.  More info

Nestola P, Silva RJ, Peixoto C, Alves PM, Carrondo MJ, Mota JP.
Robust design of adenovirus purification by two-column, simulated moving-bed, size-exclusion chromatography
Journal of Biotechnology,  213(10) 109–119 More info

Oliveira HP, Mairhofer J, Alves PM, Lara AR, Kontoravdi C
Advances in the Development of Biotherapeutics, Editorial
BioMed Research International, Volume 2015, Article ID 793876, 2 pages More info

Perdigao-Henriques, R., Petrocca, F., Altschuler, G., Thomas, M.P., Le, M.T., Tan, S.M., Hide, W., and Lieberman, J.
miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes
Oncogene 35(2):158-72 More info

Piersanti S, Burla R, Licursi V, Brito C, La Torre M, Alves PM, Simão D, Monttini C, Salinas S, Negri R, Tagliafico E, Kremer EJ, Saggio I
Transcriptional response of human neurospheres to helper-dependent CAV-2 vectors: activation of DNA damage response, modulation of microtubule motors and centromeric proteins
Plos One, July 24 More info

Rebelo SP, Costa R, Silva MM, Marcelino P, Brito C, Alves PM
Three-dimensional co-culture of human hepatocytes and mesenchymal stem cells: improved functionality in long-term bioreactor cultures.
J Tissue Eng Regen Med [Epub ahead of print] More info

Rebelo, S.P., Costa, R., Sousa, M.F., Brito, C., and Alves, P.M.
Establishing Liver Bioreactors for In Vitro Research
Methods in Molecular Biology 1250, 189-202. More info

Rodrigues A F, Guerreiro M R, Formas-Oliveira A S, Fernandes P, Blechert A-K, Genzel Y, Alves P M, Hu W S, Coroadinha A S.
Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK
Biotechnol Bioeng. Jul 1 More info

Rodrigues AF, Formas-Oliveira AS, Guerreiro M, Tomas H, Alves PM and Coroadinha AS
Single step cloning-screening method: a new tool for developing and studying high titer viral vector producer cells
Gene Ther, May 4 More info

Rodrigues AF, Soares HR, Guerreiro MR, Alves PM, Coroadinha AS.
Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology
Biotechnol J. 2015 Sep;10(9):1329-44 More info

Silva AC, Simão D, Sousa MFQ, Küppers C, Lucas T, Cruz P, Carrondo MJ, Kochanek S, Alves PM
Human Amniocyte-derived Cells for the Production of Adenoviral Vectors
Biotechnology Journal, May;10(5):760-71 More info

Silva MM, Rodrigues AF, Correia C, Sousa MQF, Brito C, Coroadinha AS, Serra M, Alves PM
Robust expansion of human pluripotent stem cells: integration of bioprocess design with transcriptomic and metabolomic characterization
Stem Cells and Translational Medicine, Jul;4(7):731-42 More info

Simao D, Pinto C, Piersanti S, Weston A, Peddie CJ, Bastos AEP, Licursi V, Schwarz, S. C., Collinson, L. M., Salinas S, Serra M, Teixeira AP, Saggio I, Lima PA, Kremer EJ, Schiavo G, Brito C & Alves PM
Modeling Human Neural Functionality In Vitro: Three-Dimensional Culture for Dopaminergic Differentiation
Tissue Engineering Part A 21, 654-668. More info

Sousa MFQ, Silva MM, Girous D, Hashimura Y, Wesselschmidt R, Lee B, Roldão A, Carrondo MJT, Alves PM, Serra M
Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, vertical-wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture pro
Biotechnology Progress, 31(6):1600-12 More info

Suter-Dick L, Alves PM, Blaauboer BJ, Bremm KD, Brito C, Coecke S, Flick B, Fowler P, Hescheler J, Ingelman-Sundberg M, Jennings P, Kelm JM, Manou I, Mistry P, Moretto A, Roth A, Stedman D, van de Water B, Beilmann M
Stem Cell-Derived Systems in Toxicology Assessment
Stem Cells Dev. 24(11):1284-96 More info

Terrasso AP, Pinto C, Serra M, Filipe A, Almeida S, Ferreira AL, Pedroso P, Brito C, Alves PM
Novel scalable 3D cell based model for in vitro neurotoxicity testing: Combining human differentiated neurospheres with gene expression and functional endpoints
J Biotechnol 205, 82-92. More info

Vlaming M, van Duijn E, Dillingh MR, Brands R, Windhorst AD, Hendrikse NH, Bosgra S, Burggraaf J, de Koning MC, Fidder A, Mocking J, Sandman H, de Ligt R, Fabriek BO, Pasman WJ, Seinen W, Alves T, Carrondo M, Peixoto C, Peeters P, Vaes W
Microdosing of a carbon-14 labeled protein in healthy volunteers accurately predicts its pharmacokinetics at therapeutic dosages
Clin Pharmacol Ther. Apr 13 More info


Correia C, Serra M, Espinha N, Sousa M, Brito C, Burkert K, Zheng Y, Hescheler J, Carrondo MJ, Sarić T, Alves PM.
Combining Hypoxia and Bioreactor Hydrodynamics Boosts Induced Pluripotent Stem Cell Differentiation Towards Cardiomyocytes.
Stem Cell Reviews and Reports. Published on line 15 July 2014. More info

Duarte TM, Carinhas N, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP
Metabolic responses of CHO cells to limitation of key amino acids.
Biotechnol Bioeng, 111(10): 2095-2106 More info

Fernandes F, Dias MM, Vidigal J, Patrone M, Teixeira AP, Alves PM
Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange
J Biotechnol. 2014 Feb 10;171:34-8 More info

Gualda EJ, Simão D, Pinto C, Alves PM, Brito C.
Imaging of human differentiated 3D neural aggregates using light sheet fluorescence.
Frontiers in Cellular Neuroscience. 8:221.  More info

Hickman J, Graeser R, De Hoogt R, Vidic S, Brito C, Gutekunst M, Van der Kuip H.
Capturing tumor complexity in vitro/ex vivo: three dimensional models of cancer for pharmacology and cancer cell biology.
Biotechnology Journal 9: 1115-28. 

Monteiro F, Bernal V, Saelens X, Lozano AB, Bernal C , Sevilla A, Carrondo MJT, Alves PM.
Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system’s Productivity.
Biotechnol Bioeng. 111(4): 816–28. More info

Nestola P, Martins DL, Peixoto C, Roederstein S, Schleuss T, Alves PM, Mota JPB, Carrondo MJ
Evaluation of Novel Large Cut-Off Ultrafiltration Membranes for Adenovirus Serotype 5 (Ad5) Concentration
PLoS ONE, 9(12):-115802 More info

Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB.
Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography.
Journal of Chromatography A,1347: 111–121. More info

Nestola P, Villain L, Peixoto C, Martins DL, Alves PM, Carrondo MJT, Mota JPB.
Impact of grafting on the design of new membrane adsorbers for adenovirus purification.
Journal of Biotechnology, 181: 1–11 More info

Pais DAM, Carrondo MJT, Alves PM, Teixeira AP.
Towards real-time monitoring of therapeutic protein quality in mammalian cell processes.
Current Opinion in Biotechnology. Jul 15;30C:161-167 More info

Patrone M, Carinhas N, Sousa MQ, Peixoto C, Ciferri C, Carfi A, Alves PM
Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
PLoS One. Mar 4;9(3):e90753.  More info

Piersanti, S., Licursi, V., Brito, C., Burla, R., Alves, P., Simao, D., Devau, G., Mottini, C., Negri, R., Tagliafico, E., Kremer, E. J. & Saggio, I.
High Throughput Comparative Analysis of the Response of Human and Nonhuman Primate Neurons To Viral Vectors
Molecular Therapy 22, S192-S192. More info

Rebelo SP, Costa R, Estrada M, Shevchenko V, Brito C, Alves PM.
HepaRG microencapsulated spheroids in DMSO‑free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism.
Archives of Toxicology. Published online 09 August 2014.  More info

Rodrigues AF, Carrondo MJ, Alves PM, Coroadinha AS
Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells
Trends in Biotechnology, 32(12):602-7 More info

Santo VE, Estrada M, Veloso S, Sousa MFQ, Van der Kuip H, Oren M, Boghaert ER, Alves PM & Brito C
Recapitulation of non-small-cell lung carcinoma microenvironment in perfusion bioreactor cultures: the impact of hypoxia on tumour-stroma crosstalk.
European Journal of Cancer 50, S83-S83. More info

Sarmento L, Póvoa V, Nascimento R, Real G, Antunes I, Martins L, Moita M, Alves PM, Abecasis M, Moita L, Parkhouse R, Meijerink J and Barata JT.
CHK1 overexpression in T-cell acute lymphoblastic leukemia is essential for proliferation and survival by preventing excessive replication
Oncogene. 2014 Aug 18;0.  More info

Silva AC, Yami M, Libeau G, Carrondo MJ, Alves PM.
Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia.
Vaccine, 32(24): 2878–81 More info


Fernandes F, Teixeira AP, Carinhas N, Carrondo MJT, Alves PM
Insect cells as production platform of complex VLPs
Expert Rev Vaccines. Feb,12(2):225-36 More info

Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM
Bioprocess development for canine adenovirus type 2 vectors
Gene Ther. Apr,20(4):353-60 More info

Fernandes P, Santiago VM, Rodrigues AF, Tomás H, Kremer EJ, Alves PM, Coroadinha AS
Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines
PLoS One. 2013,8(4):e60342. Epub 2013 Apr 2 More info

Lamego J, Cunha B, Peixoto C, Sousa MF, Alves PM, Simplicio AL, Coroadinha AS
Carboxylesterase 2 production and characterization in human cells: new insights into enzyme oligomerization and activity
Appl Microbiol Biotechnol. Feb,97(3):1161-73 More info

Carinhas N, Duarte TM, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP.
Metabolic signatures of GS CHO cell clones associated with butyrate treatment and culture phase transition.
Biotechnol Bioeng, 110 (12): 3244-57.   More info

Fonseca CP, Fonseca LL, Montezinho LP, Alves PM, Santos H, Castro MM, Geraldes CF.
(23)Na multiple quantum filtered NMR characterisation of Na(+) binding and dynamics in animal cells: a comparative study and effect of Na(+)/Li (+) competition.
Eur Biophys J. Apr 6.  42(7): 503-519  More info

Rodrigues AF, Formas-Oliveira AS, Bandeira, VS, Alves PM, Hu WS, Coroadinha AS.
Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineering.
Metab. Eng. 20: 131-137.  More info

Simplício AL, Coroadinha AS, Gilmer JF, Lamego J.
A methodology for detection and quantification of esterase activity.
Methods Mol Biol. 2013;984:309-19.  More info

Vidigal J, Dias MM, Fernandes F, Patrone M, Bispo C, Andrade C, Gardner R, Carrondo MJT, Alves PM, Teixeira AP
A cell sorting protocol for selecting high-producing sub-populations of Sf9 and High FiveTM cells,
J Biotech. 168 (4): 436-49.   More info


Almeida AS, Queiroga CS, Sousa MF, Alves PM, Vieira HL
Carbon monoxide modulates apoptosis by reinforcing oxidative metabolism in astrocytes: role of BCL-2
J Biol Chem, 287 (14): 10761-70 More info

Bandeira V, Peixoto C, Rodrigues AF, Cruz PE, Alves PM, Coroadinha AS, Carrondo MJ
Downstream processing of lentiviral vectors: releasing bottlenecks
Hum Gene Ther Methods, 23(4):255-63 More info

Brito C, Simão D, Costa I, Malpique R, Pereira CI, Fernandes P, Serra M, Schwarz SC, Schwarz J, Kremer EJ, Alves PM
Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells
Methods, 56(3):452-60 More info

Carinhas N, Oliveira R, Alves PM, Carrondo MJ, Teixeira AP
Systems biotechnology of animal cells: the road to prediction
Trends Biotechnol, 30(7):377-85 More info

Carrondo MJ, Alves PM, Carinhas N, Glassey J, Hesse F, Merten OW, Micheletti M, Noll T, Oliveira R, Reichl U, Staby A, Teixeira AP, Weichert H, Mandenius CF
How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology?
Biotechnol J, Epub ahead of print More info

Fernandes F, Vidigal J, Dias M, Prather KL, Coroadinha AS, Teixeira AP, Alves PM
Flipase-mediated cassette exchange in Sf9 insect cells for stable gene expression
Biotechnol Bioeng, 109(11):2836-44 More info

Leite SB, Wilk-Zasadna I, Zaldivar Comenges JM, Airola E, Reis-Fernandes MA, Mennecozzi M, Guguen-Guillouzo C, Chesne C, Gouillou C, Alves PM, Coecke S
3D HepaRG model as an attractive tool for toxicity testing
Toxicol Sci, Epub ahead of print More info

Malpique R, Tostões R, Beier AF, Serra M, Brito C, Schulz JC, Björquist P, Zimmermann H, Alves PM
Surface-based cryopreservation strategies for human embryonic stem cells: A comparative study
Biotechnol Prog, 28(4):1079-87 More info

Marcelino I, Almeida AM, Brito C, Meyer D, Barreto M, Sheikboudou C, Franco CF, Martinez D, Lefrançois T, Vachiéry N, Carrondo MJ, Coelho A, Alves PM
Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins
Vet Microbiol, 156 (2012) 305–314 More info

Monteiro F, Carinhas N, Carrondo MJT, Bernal V and Alves PM.
Toward system-level understanding of baculovirus–host cell interactions: from molecular fundamental studies to large-scale proteomics approaches
Front. Microb. doi: 10.3389/fmicb.2012.00391 More info

Queiroga CS, Almeida AS and Vieira HL
Carbon monoxide targeting mitochondria
Biochem Res Int, 2012:749845 More info

Queiroga CS, Tomasi S, Wideroe M, Alves PM, Vercelli A, Vieira HL
Preconditioning triggered by Carbon Monoxide (CO) provides neuronal protection following perinatal hypoxia-ischemia
Plos One, 7(8): e42632 More info

Rodrigues AF, Amaral AI, Veríssimo V, Alves PM, Coroadinha AS
Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production
Biotechnol Bioeng, 109 (5): 1269-79 More info

Roldão A, Mellado MC, Lima JC, Carrondo MJ, Alves PM, Oliveira R
On the effect of thermodynamic equilibrium on the assembly efficiency of complex multi-layered virus-like particles (VLP): the case of rotavirus VLP
PLoS Comput Biol, 8(2): e1002367 More info

Serra M, Brito C, Correia C, Alves PM
Process engineering human pluripotent stem cells for clinical applications
Trends Biotechnol, 30(6):350-9 More info

Tavares L, Figueira I, McDougall GJ, Vieira HL, Stewart D, Alves PM, Ferreira RB, Santos CN
Neuroprotective effects of digested polyphenols from wild blackberry species
Eur J Nutr More info

Tostões R, Leite SB, Serra M, Jensen J, Björquist P, Carrondo MJ, Brito C, Alves PM
Human liver cell spheroids in extended perfusion bioreactor culture for repeated dose drug testing
Hepatology, 55(4): 1227-36 More info


Amaral AI , Teixeira AP, Sonnewald U, Alves PM
Estimation of intracellular fluxes in cerebellar neurons after hypoglyce: importance of the pyruvate recycling pathway and glutamine oxidation
J Neurosci Res, 89(5)-700:10 More info

Amaral IA, Teixeira AP, Håkonsen BI, Sonnewald U, Alves PM
A comprehensive metabolic profile of cultured astrocytes using isotopic transient metabolic flux analysis and 13C-labelled glucose
Front Neuroenerg, 3:5 More info

Carinhas N, Bernal V, Teixeira AP, Carrondo MJ, Alves PM, Oliveira R
Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products
BMC Syst Biol,5:34 More info

Carinhas N, Robitaille AM, Moes S, Carrondo MJ, Jenoe P, Oliveira R, Alves PM
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection
Plos One, 6(10):e26444 More info

Carrondo MJ, Panet A, Wirth D, Coroadinha AS, Cruz P, Falk H, Schucht R, Dupont F, Geny-Fiamma C, Merten OW, Hauser H
Integrated strategy for the production of therapeutic retroviral vectors
Hum Gene Ther, 22(3): 370-79 More info

Carvalhal AV, Santos SS, Carrondo MJ
Extracellular purine and pyrimidine catabolism in cell culture
Biotechnol Prog,27:1373-82 More info

Cruz PE, Rodrigues T, Carmo M, Wirth D, Amaral AI, Alves PM, Coroadinha AS
Manufacturing of retroviruses
Methods Mol Biol, 737:157-82 More info

Gama-Norton L, Botezatu L, Herrmann S, Schweizer M, Alves PM, Hauser H, Wirth D
Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells
Hum Gene Ther, 22(10):1269-79 More info

Lamego J, Coroadinha AS, Simplicio AL
Detection and quantification of carboxylesterase 2 activity by capillary electrophoresis
Anal Chem, 83(3):881–887 More info

Leite SB, Teixeira AP, Miranda JP, Tostoes RM, Clemente JJ, Sousa MF, Carrondo MJ, Alves PM
Merging bioreactor technology with 3D hepatocyte-fibroblast culturing approaches: improved in vitro models for Toxicological applications
Toxicol in Vitro, 25(4):825-32 More info

Lesch HP, Laitinen A, Peixoto C, Vicente T, Makkonen ME, Laitinen L, Pikkarainen JT, Samaranayake H, Alves PM, Carrondo MJ, Ylä-Herttuala S, Airenne KJ
Production and Purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors
Gene Ther, 18:531-38 More info

Queiroga CS, Almeida AS, Alves PM, Brenner C, Vieira HL
Carbon monoxide prevents hepatic mitochondrial membrane permeabilization
BMC Cell Biol 12:10 More info

Real G, Monteiro F, Burger C, Alves PM
Improvement of lentiviral transfer vectors using cis-acting regulatory elements for increased gene expression
Appl Microbiol Biotechnol,91:1581-91 More info

Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS
Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition
Biotechnol Bioeng, 108(11):2623-33 More info

Roldão A, Vicente T, Peixoto C, Carrondo MJ, Alves PM
Quality control and analytical methods for baculovirus based products
J Invertebr Pathol, 107:S94-S105 More info

Santos SS, Sonnewald U, Carrondo MJ, Alves PM
The role of glia in neuronal recovery following anoxia: in vitro evidence of neuronal adaptation
Neurochem Int,58:665-75 More info

Serra M, Correia C, Malpique R, Brito C, Jensen J, Bjorquist P, Carrondo MJ, Alves PM
Microencapsulation technology: a powerful tool for integrating expansion and cryopreservation of human embryonic stem cells
Plos One, 6(8) e23212 More info

Silva AC, Carrondo MJ, and Alves PM
Strategies for improved stability of Peste des Petits Ruminants vaccine
Vaccine, 29:4983-91 More info

Tavares L, Alves PM, Ferreira RB, Santos CN
Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma
BMC Res Notes, 1:140 More info

Teixeira AP, Dias JM, Carinhas N, Sousa MF, Clemente JJ, Cunha AE, Von Stosch M, Alves PM, Carrondo MJ, Oliveira R
Cell functional enviromics: unravelling the function of environmental factors
BMC Syst Biol,5:92 More info

Teixeira AP, Duarte TM, Carrondo MJ, Alves PM
Synchronous fluorescence spectroscopy as a novel tool to enable PAT applications in bioprocesses
Biotechnol Bioeng, 108(8):1852-61 More info

Teixeira AP, Duarte TM, Oliveira R, Carrondo MJ, Alves PM
High-throughput analysis of animal cell cultures using two dimensional fluorometry
J Biotechnol, 151:255-60 More info

Tostões RM, Leite SB, Miranda JP, Sousa MF, Wang DIC, Carrondo MJ, Alves PM
Perfusion of 3D encapsulated hepatocytes-a synergistic effect enhancing long-term functionality in bioreactors
Biotechnol Bioeng,108: 41-49 More info

Vicente T, Fáber R, Alves PM, Carrondo MJ, Mota JP
Impact of ligand density on the optimization of ion-exchange membrane chromatography for viral vector purification
Biotechnol Bioeng, 108(6):1347-59 More info

Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ
Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: current advances
Biotechnol Adv,29: 869-78 More info

Vicente T, Roldão A, Peixoto C, Carrondo MJ, Alves PM
Large-scale production and purification of VLP-based vaccines
J Invertebr Pathol, 107:S42-S48 More info

Vieira HL, Alves PM, Vercelli A
Modulation of neuronal stem cell differentiation by hypoxia and reactive oxygen species
Prog Neurobiol, 93:444-55 More info

Mandenius CF, Andersson TB, Alves PM, Batzl-Hartmann C, Björquist P, Carrondo MJT, Chesne C, Coecke S, Edsbagge J, Fredriksson JM, Gerlach JC, Heinzle E, Ingelman-Sundberg M, Johansson I, Küppers-Munther B, Müller-Vieira U, Noor F, Zeilinger K,
Toward Preclinical Predictive Drug Testing for Metabolism and Hepatotoxicity by Using In Vitro Models Derived from Human Embryonic Stem Cells and Human Cell Lines - A Report on the Vitrocellomics EU-p
ATLA 39:147:171

Roldão A, Vicente T, Peixoto C, Carrondo MJT, Alves PM
Quality control for baculovirus-based products
Journal of Invertebrate Pathology, (Baculovirus in Medicine - Special Issue), 107:S94-105.


Adakal H, Stachurski F, Konkobo M, Zoungrana S, Meyer DF, Pinarello V, Aprelon R, Marcelino I, Alves PM, Martinez D, Lefrancois T, Vachiéry N
Efficiency of inactivated vaccines against heartwater in Burkina Faso: Impact of Ehrlichia ruminantium genetic diversity
Vaccine, 28(29):4573-80 More info

Amaral AI, Teixeira AP, Martens S, Sousa MF, Bernal V, Alves PM
Metabolic alterations induced by ischemia in primary cultures of astrocytes: merging 13C NMR spectroscopy and metabolic flux analysis
J Neurochem, 113(3):735-48 More info

Bernal V, Monteiro F, Carinhas N, Ambrósio R, Alves PM
An integrated analysis of enzyme activities, cofactor pools and metabolic fluxes in baculovirus-infected Spodoptera frugiperda Sf9 cells
J Biotechnol, 150:332-42 More info

Carinhas N, Bernal V, Monteiro F, Carrondo MJ, Oliveira R, Alves PM
Improving baculovirus production at high cell density through manipulation of energy metabolism
Metabol Eng, 12:39-52 More info

Coroadinha AS, Gama-Norton L, Amaral AI, Hauser H, Alves PM, Cruz PE
Production of retroviral vectors
Curr Gene Ther, 10(6):456-73

Fortalezas S, Tavares L, Pimpão R, Tyagi M, Pontes V, Alves PM, McDougall G, Stewart D, Ferreira RB, Santos CN
Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo)
Nutrients, 2(2):214-29 More info

Franco CF, Mellado MC, Alves PM, Coelho AV
Monitoring virus-like particle and viral protein production by intact cell MALDI-TOF mass spectrometry
Talanta, 80(4):1561-8 More info

Gama-Norton L, Herrmann S, Schucht R, Coroadinha AS, Löw R, Alves PM, Bartholomae CC, Schmidt M, Baum C, Schambach A, Hauser H, Wirth D
Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines
Hum Gene Ther, 21(8):979-91 More info

Malpique R, Osório LM, Ferreira DS, Ehrhart F, Brito C, Zimmermann H, Alves PM
Alginate encapsulation as a novel strategy for the cryopreservation of neurospheres
Tissue Eng Part C Methods, 16(5):965-77 More info

Miranda JP, Rodrigues A, Tostões RM, Leite SB, Zimmerman H, Carrondo MJ, Alves PM
Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors
Tissue Eng Part C Methods, 16(6):1223-32 More info

Queiroga CS, Almeida AS, Martel C, Brenner C, Alves PM, Vieira HL
Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilisation and apoptosis
J Biol Chem, 285(22):17077-17088 More info

Roldão A, Mellado MC, Castilho LR, Carrondo MJ, Alves PM
Virus-like particles in vaccine development
Expert Rev Vaccines, 9(10):1149-76 More info

Serra M, Brito C, Sousa MF, Jensen J, Tostões RM, Clemente JJ, Hyllner J, Strehl R, Carrondo MJ, Alves PM
Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control
J Biotechnol, 148(4):208-15 More info

Silva AC, Peixoto C, Lucas T, Kuppers C, Cruz PE, Alves PM, Kochanek S
Adenovirus vector production and purification
Curr Gene Ther, 10(6):437-55

Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ
Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance
J Chromatogr A, 1217:2032-41 More info

Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ
Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance
J Biotechnol, 148(4):171-81 More info

Vicente T, Peixoto C, Alves PM, Carrondo MJ
Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development
J Chromatogr A, 1217:3754-64 More info

Vieira HL, Pereira AC, Peixoto C, Moraes RH, Alves PM, Mendonça RZ
Improvement of recombinant protein production by an anti-apoptotic protein from hemolymph of Lonomia obliqua
Cytotechnology, 62(6):547-55 More info

Bernal V, Monteiro F, Carinhas N., Ambrósio R, Carrondo MJT, Alves PM
Metabolic burden in baculovirus‐infected Spodoptera frugiperda Sf9 cells
Journal Biotechnology, 150(3):332-42. More info

Matias AA, Serra AT, Silva AC, Perdigão R, Ferreira TB, Marcelino I, Silva S, Coelho AV, Alves PM, Duarte CM
Portuguese winemaking residues as a potential source of natural anti-adenoviral agents
Int. J Food Sci Nutr, 61(4):357-68 More info


Bannenberg GL, Vieira HL
Therapeutic applications of the gaseous mediators carbon monoxide and hydrogen sulfide
Expert Opin Ther Pat, 19:663-82 More info

Bernal V, Carinhas N, Yokomizo A, Carrondo MJ, Alves PM
Cell density effect in the Baculovirus-Insect cells system: a quantitative analysis of energetic metabolism
Biotechnol Bioeng, 104(1):162-80 More info

Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM
Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange
Appl Microbiol Biotechnol, 81(6):1041-49 More info

Carmo M, Alves A, Rodrigues AF, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE
Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer
J Gene Med, 11:670-78 More info

Carmo M, Dias JD, Panet A, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE
Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors
Hum Gene Ther, 20(10):1168-76 More info

Ferreira TB, Perdigão R, Silva AC, Zhang C, Aunins JG, Carrondo MJ, Alves PM
293 cell cycle synchronisation in adenovirus vector production
Biotechnol Prog, 25(1):235-43 More info

Güll I, Alves PM, Gabor F, Wirth M
Viability of human adenocarcinoma cell line Caco-2: influence of cryoprotectant, freezing rate, and storage temperature
Sci Pharm, 77:133-41 More info

Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM
Cryopreservation of adherent cells: strategies to improve cell viability and function after thawing
Tissue Eng Part C Methods, 15(3):373-86 More info

Mellado MC, Mena JA, Lopes A, Ramirez OT, Carrondo MJ, Palomares LA, Alves PM
Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles
Biotechnol Bioeng, 104(4):674-86 More info

Miranda JP, Leite SB, Muller-Vieira U, Rodrigues A, Carrondo MJ, Alves PM
Toward extended functional hepatocyte in vitro culture
Tissue Eng Part C Methods, 15:1-11 More info

Rodrigues AF, Carmo M, Alves PM, Coroadinha AS
Retroviral vector production under serum deprivation: the role of lipids
Biotechnol Bioeng, 104(6):1171-81 More info

Roldão A, Oliveira R, Carrondo MJ, Alves PM
Error assessment in recombinant baculovirus titration: evaluation of different Methods
J Virol Methods, 159:69-80 More info

Serra M, Brito C, Costa E, Sousa MF, Alves PM
Integrating human stem cell expansion and neuronal differentiation in bioreactors
BMC Biotechnol, 9:82 More info

Serra M, Brito C, Leite SB, Gorjup E, von Briesen H, Carrondo MJ, Alves PM
Stirred bioreactors for the expansion of adult pancreatic stem cells
Ann Anat, 191:104-15 More info

Teixeira AP, Oliveira R, Alves P, Carrondo MJ
Advances in on-line monitoring and control of mammalian cell cultures: supporting the PAT initiative
Biotechnol Adv, 27:726-32 More info

Teixeira AP, Portugal CA, Carinhas N, Dias JM, Crespo JP, Alves PM, Carrondo MJ, Oliveira R
In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures
Biotechnol Bioeng, 102(4):1098-106 More info

Vicente T, Peixoto C, Carrondo MJ, Alves PM
Virus production for clinical gene therapy
Methods Mol Biol, 542:447-70 More info

Vicente T, Peixoto C, Carrondo MJ, Alves PM
Purification of recombinant baculoviruses for gene therapy using membrane processes
Gene Ther, 16:766-775 More info

Vieira HL, Cunha L, Goldmacher VS, Alves PM
The effect of the cell death suppressor vMIA on the production of a recombinant protein in the adenovirus-293 expression system
Protein Expr Purif, 64(2):179-84 More info


Amaral AI, Coroadinha AS, Merten O-W, Alves PM
Improving retroviral vectors production: role of carbon sources in lipid biosynthesis
J Biotechnol, 138: 57-66 More info

Carmo M, Panet A, Carrondo MJ, Alves PM, Cruz PE
From retroviral vector production to gene transfer: spontaneous inactivation is caused by loss of reverse transcription capacity
J Gene Med, 10:383-91 More info

Carrondo MJ, Merten OW, Haury M, Alves PM, Coroadinha AS
Impact of retroviral vector components stoichiometry on packaging cell lines: effects on productivity and vector quality
Hum Gene Ther, 19:199-210 More info

Clemente JJ, Monteiro SMS, Carrondo MJ, Cunha AE
Predicting sporulation events in a bioreactor using an electronic nose
Biotechnol Bioeng, 101(3):545-52 More info

Gouveia RM, Gomes CM, Sousa MF, Alves, PM, Costa J
Kinetic analysis of L1 homophilic interaction: role of the first four Ig domains and implications on L1-L1 binding mechanism
J Biol Chem, 283(42):28038-47 More info

Mellado MC, Franco C, Coelho AV, AlvesPM, Simplício AL
Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles
J Chromatogr A, 1192:166-72 More info

Mellado MC, Peixoto C, Cruz PE, Carrondo MJ, Alves PM
Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly
J Chromatogr B, 874:89-94 More info

Peixoto C, Ferreira TB, Sousa MF, Carrondo MJ, Alves PM
Towards purification of adenovirus vectors based on membrane technology
Biotechnol Prog, 24:1290-96 More info

Petrovski Z, Norton de Matos MR, Braga SS, Pereira CC, Matos ML, Gonçalves IS, Pillinger M, Alves PM, Romão CC
Synthesis, characterization and antitumor activity of 1,2-disubstituted ferrocenes and cyclodextrin inclusion complexes
J Organomet Chem, 693:675-84 More info

Rodrigues T, Alves A, Lopes A, Carrondo MJ, Alves PM, Cruz PE
Removal of envelope protein-free retroviral vectors by anion exchange chromatography to improve product quality
J Sep Sci, 31:3509-18 More info

Roldão A, Carrondo MJ, Alves PM, Oliveira R
Stochastic simulation of protein expression in the baculovirus/insect cell system
Comp Chem Eng, 32:68-77 More info

Silva AC, Delgado I, Sousa MF, Carrondo MJ, Alves PM
Scalable culture systems using different cell lines for the production of Peste des Petits Ruminants vaccine
Vaccine, 26:3305-11 More info

Teixeira AP, Santos SS, Carinhas N, Oliveira R, Alves PM
Combining metabolic flux analysis tools and 13C-NMR to estimate intracellular fluxes of cultured astrocytes
Neurochem Int, 52:478-86 More info

Vicente T, Sousa MF, Peixoto C, Mota JP, Alves PM, Carrondo MJ
Anion exchange membrane chromatography for purification of rotavirus-like particles
J Membr Sci, 311:270-83 More info

Vieira HL, Queiroga CS, Alves PM
Preconditioning induced by Carbon Monoxide provides neuronal protection against apoptosis
J Neurochem, 107:375-84 More info


Bento I, Peixoto C, Zaitsev VN, Lindley P
Ceruloplasmin revisited: structural and functional roles of various metal cation-binding sites
Acta Crystallogr D Biol Crystallogr, 63(2):240-48 More info

Faburay B, Geysen D, Ceesay A, Marcelino I, Alves PM, Taoufik A, Postigo M, Bell-Sakyi L, Jongejan F
Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines
Vaccine, 25(46):7939-47 More info

Ferreira TB, Carrondo MJ, Alves PM
Effect of ammonia production on intracellular pH consequent effect on adenovirus vector production
J Biotechnol, 129(3):433-38 More info

Gouveia RM, Morais VA, Peixoto C, Sousa MF, Regalla M, Alves PM, Costa J
Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells
Protein Expr and Purif, 52:182-93 More info

Malpique R, Katsen-Globa A, Carrondo MJ, Zimmermann H, Alves PM
Cryopreservation in micro-volumes: impact upon Caco-2 colon adenocarcinoma cells proliferation and differentiation
Biotechnol Bioeng, 98:155-66 More info

Marcelino I, Vachiéry N, Amaral AI, Roldão A, Lefrançois T, Carrondo MJ, Alves PM, Martinez D
Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater
Vaccine, 25(26):4903-13 More info

Peixoto C, Marcelino I, Amaral AI, Carrondo MJ, Alves PM
Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater
Proc Biochem, 42(7):1084-89 More info

Peixoto C, Sousa MF, Silva AC, Carrondo MJ, Alves PM
Downstream processing of triple-layered rotavirus like particles
J Biotechnol, 127:452-61 More info

Rodrigues T, Carrondo MJ, Alves PM, Cruz PE
Purification of retroviral vectors for clinical application: biological implication and technological challenges
J Biotechnol, 127:520-41 More info

Rodrigues T, Carvalho A, Carmo M, Carrondo MJ, Alves PM, Cruz PE
Scaleable purification process for gene therapy retroviral vectors
J Gene Med, 9(4):233-43 More info

Roldão A, Vieira HL, Charpilienne A, Poncet D, Roy P, Carrondo MJ, Alves PM, Oliveira R
Modeling rotavirus-like particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production
J Biotechnol, 128(4):875-94 More info

Santos SS, Leite SB, Sonnewald U, Carrondo MJ, Alves PM
Stirrer vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics
J Neurosci Res, 85(15):3386-97 More info

Serra M, Leite SB, Brito C, Costa J, Carrondo MJ, Alves PM
Novel culture strategy for human stem cell proliferation and neuronal differentiation
J Neurosci Res, 85(16):3557-66 More info

Teixeira A, Alves C, Alves PM, Carrondo MJ, Oliveira R
Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control
BMC Bioinformatics, 8(1):30 More info

Teixeira AP, Carinhas N, Dias JM, Cruz PE, Alves PM, Carrondo MJ, Oliveira R
Hybrid semi-parametric mathematical systems: bridging the gap between biology and process engineering
J Biotechnol, 132:418-25 More info

Wirth D, Gama-Norton L, Riemer P, Sandhu U, Schucht R, Hauser H
Road to precision: recombinase-based targeting technologies for genome engineering
Curr Opin Biotechnol, 18:411–19 More info


Antonis AF, Bruschke CJ, Rueda P, Maranga L, Casal JI, Vela C, Hilgers LA, Belt PB, Weerdmeester K, Carrondo MJ, Langeveld JP
A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure
Vaccine, 24(26):5481-90 More info

Brennan L, Alves PM, Hewage C, Malthouse JG, McBean GJ
Impact of gliotoxin L-serine-O-suphate on cellular metabolism in cultured astrocytes
Neurochem Int, 48:739-45 More info

Carmo M, Faria TQ, Falk H, Coroadinha AS, Teixeira M, Merten OW, Geny-Fiamma C, Alves PM, Danos O, Panet A, Carrondo MJ, Cruz PE
Relationship between retroviral vector membrane and vector stability
J Gen Virol, 87(5):1349-56 More info

Coroadinha AS, Alves PM, Santos SS, Cruz PE, Merten, OW, Carrondo MJ
Retrovirus producer cell line metabolism: implications on viral productivity
Appl Microbiol Biotechnol. 72(6):1125-35 More info

Coroadinha AS, Ribeiro J, Roldão A, Cruz PE, Alves PM, Merten O-W, Carrondo MJ
Effect of medium sugar source on the production of retroviral vectors for gene therapy
Biotechnol Bioeng, 94(1):24-36 More info

Coroadinha AS, Schucht R, Gama-Norton L, Wirth D, Hauser H, Carrondo MJ
The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchange
J Biotechnol, 124(2):457-68 More info

Coroadinha AS, Silva AC, Pires E, Coelho A, Alves PM, Carrondo MJ
Effect of osmotic pressure on the production of retroviral vectors: enhancement in vector stability
Biotechnol Bioeng, 94(2):322-9 More info

Cruz PE, Silva AC, Roldao A, Carmo M, Carrondo MJ, Alves PM
Screening of novel excipients for improving the stability of retroviral and adenoviral vectors
Biotechnol Prog, 22(2):568-76 More info

Lameiro MH, Lopes A, Martins LO, Alves PM, Melo E
Incorporation of a model protein into chitosan-bile salt microparticles
Int J Pharm, 312:119-30 More info

Lameiro MH, Malpique R, Silva AC, Alves PM, Melo E
Encapsulation of adenoviral vectors into chitosan-bile salt microparticles for mucosal vaccination
J Biotechnol, 126:152-62 More info

Marcelino I, Sousa MF, Veríssimo C, Cunha A, Carrondo MJ, Alves PM
Process development for the mass production of Ehrlichia ruminantium
Vaccine, 24(10):1716-25 More info

Peixoto C, Ferreira TB, Carrondo MJ, Cruz PE, Alves PM
Purification of adenoviral vectors using expanded bed chromatography
J Virol Methods,132:121-6 More info

Rodrigues T, Carvalho A, Roldao A, Carrondo MJ, Alves PM, Cruz PE
Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors
J Chromatogr B, 837(1-2):59-68 More info

Roldão A, Vieira HL, Alves PM, Oliveira R, Carrondo MJ
Intracellular dynamics in rotavirus-like particles production: evaluation of multigene and monocistronic infection strategies
Proc Biochem, 41:2188-99 More info

Santos SS, Gibson GE, Cooper AJ, Denton TT, Thompson CM, Bunik VI, Alves PM, Sonnewald U
Inhibitors of the a-ketoglutarate dehydrogenase complex alter [1-13C]Glucose and [U-13C]Glutamate metabolism in cerebellar granule neurons
J Neurosci Res, 83:450-8 More info

Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D
A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors
Mol Ther, 14(2):285-92 More info

Teixeira AP, Clemente JJ, Cunha AE, Carrondo MJ, Oliveira R
Bioprocess iterative batch-to-batch optimization based on hybrid parametric/nonparametric models
Biotechnol Prog, 22(1):247-58 More info

Vieira HL, Pereira AC, Carrondo MJ, Alves PM
Catalase effect on apoptosis for the improvement of recombinant protein production in baculovirus-insect cell system
Bioprocess Biosyst Eng, 29(5-6):409-14 More info

Zhang C, Ferreira TB, Cruz PE, Alves PM, Haury M, Carrondo MJ
The importance of 293 cell cycle phase on adenovirus vector production
Enzyme Microb Technol, 39:1328-32 More info


Ferreira TB, Alves PM, Aunins JG,Carrondo MJ
Use of adenoviral vectors as veterinary vaccines
Gene Ther, 12(S1):73-83 More info

Ferreira TB, Ferreira AL, Carrondo MJ, Alves PM
Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production
Biotechnol Lett, 27(22): 1809-13 More info

Ferreira TB, Ferreira AL, Carrondo MJ, Alves PM
Effect of refeed strategies and non-ammoniagenic medium on adenovirus production at high cell densities
J Biotechnol, 119:272-80 More info

Fonseca LL, Monteiro MA, Alves PM, Carrondo MJ, Santos H
Cultures of rat astrocytes challenged with a steady supply of glutamate: a new model to study flux distribution in the glutamate-glutamine cycle
Glia, 51:286-96 More info

Kreij K, Mandenius CF, Clemente JJ, Cunha AE, Monteiro SM, Carrondo MJ, Hesse F, de Los Molinas MM, Wagner R, Merten OW, Gény-Fiamma C, Leger W, Wiesinger-Mayr H, Müller D, Katinger H, Mårtensson P, Bachinger T, Mitrovics J
On-line detection of microbial contaminations in animal cell reactor cultures using an electronic nose device
Cytotechnology, 48(1-3):41-58 More info

Marcelino I, Veríssimo C, Sousa MF, Carrondo MJ, Alves PM
Characterization of Ehrlichia ruminantium replication and releasekinetics in endothelial cell cultures
Vet Microbiol, 110:87-96 More info

Monteiro SM, Clemente JJ, Henriques AO, Gomes RJ, Carrondo MJ, Cunha AE
A procedure for high-yield spore production by Bacillus subtilis
Biotechnol Prog, 21(4):1026-31 More info

Oliveira R, Clemente JJ, Cunha AE, Carrondo MJ
Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation
J Biotechnol, 116(1):35-50 More info

Peixoto CC, Marcelino I, Vachiéry N, Bensaid A, Martinez D, Carrondo MJ, Alves PM
Quantification of Ehrlichia ruminantium by real time PCR
Vet Microbiol, 107:273-8 More info

Santos SS, Fonseca LL, Monteiro MA, Carrondo MJ, Alves PM
Culturing primary brain astrocytes under fully controlled environment in a novel bioreactor
J Neurosci Res, 79:26-32 More info

Souza AP, Peixoto CC, Maranga L, Carvalhal AV, Moraes RH, Mendonça RZ, Pereira CA, Carrondo MJ, Mendonça RZ
Purification and characterization of an anti-apoptotic protein isolated from Lonomia obliqua Hemolymph
Biotechnol Prog, 21:99-105 More info

Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJ, Oliveira R
Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems
J Biotechnol, 118:290-303 More info

Vieira HL, Estevão C, Roldão A, Peixoto CC, Sousa MF, Cruz PE, Carrondo MJ, Alves PM
Triple layered rotavirus VLP assembly: kinetics of vector replication, mRNA stability and recombinant protein production
J Biotechnol, 120(1):72-82 More info


Carmo M, Peixoto CC, Coroadinha AS, Alves PM, Cruz PE, Carrondo MJ
Quantitation of MLV-based retroviral vectors using real-time RT-PCR
J Virol Methods, 119:115-9 More info

Carrondo MJ, Aunins JG
Biochemical engineering
Curr Opin Biotechnol, 15(5):441-3 More info

Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJ
Methanol induction optimization for scFv antibody fragment production in Pichia pastoris
Biotechnol Bioeng, 86(4):458-67 More info

Maranga L, Cunha A, Clemente J, Cruz PE, Carrondo MJ
Scale-up of virus-like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity
J Biotechnol, 107:55-64 More info


Carvalhal AV, Marcelino I, Carrondo MJ
Metabolic changes during cell growth inhibition by p27 overexpression
Appl Microbiol Biotechnol, 63:164-73 More info

Carvalhal AV, Santos SS, Calado J, Haury M, Carrondo MJ
Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins
Biotechnol Prog, 19:69-83 More info

Maranga L, Brazão TF, Carrondo MJ
Virus like particles production at low multiplicities of infection with the baculovirus insect cell system
Biotechnol Bioeng, 84:245-53 More info

Maranga L, Mendonca RZ, Bengala A, Peixoto CC, Moraes RH, Pereira CA, Carrondo MJ
Enhancement of Sf-9 cell growth and longevity through supplementation of culture medium with hemolymph
Biotechnol Prog, 19:58-63 More info

Moreira, J. L., Miranda, P. M., Alves, P. M., Marcelino, I., and Carrondo, M. J. T
Culture methods for mass production of ruminant endothelial cells
In: B. Saha (ed.) Fermentation biotechnology. Acs symposium series No. 862. p 124-141. Oxford More info


Carvalhal A, Coroadinha AS, Alves PM, Moreira JL, Hauser H, Carrondo MJ
Metabolic changes during cell growth inhibition by the IRF-1 system
Enzyme Microb Technol, 30:95-109 More info

Cruz HJ, Conradt HS, Dunker R, Peixoto CM, Cunha AE, Thomaz M, Burger C, Dias EM, Clemente J, Moreira JL, Reike E, Carrondo MJ
Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by BHK cells
J Biotechnol, 96:169-83 More info

Cruz PE, L Maranga, MJ Carrondo
Integrated process optimization: lessons from retrovirus and virus-like particle production
J Biotechnol, 99:199-214 More info

Lopes Mde F, Leitão AL, Regalla M, Marques JJ, Carrondo MJ, Crespo MT
Characterization of a highly thermostable extracellular lipase from Lactobacillus plantarum
Int J Food Microbiol, 76(1-2):107-15 More info

Maranga L, AS Coroadinha, MJ Carrondo
Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: effects on baculovirus adsorption and infection kinetics
Biotechnol Prog, 18:855-61 More info

Maranga L, PE Cruz, JG Aunins, MJ Carrondo
Production of core and virus-like particles with baculovirus infected insect cells
Adv Biochem Eng Biotechnol, 74:183-206 More info

Maranga L, Rueda P, Antonis AF, Vela C, Langeveld JP, Casal JI, Carrondo MJ
Large scale production and downstream processing of a recombinant porcine parvovirus vaccine
Appl Microbiol Biotechnol, 59:45-50 More info


Barbotin, J.-N., J.-C. Portais, P. M. Alves, and H. Santos, 261 pp. 54 Springer, Ringbuch
Nmr and immobilized cells
In: W. R (ed.) Immobilized cells - springer lab manual No. 261. p 54. Springer, Ringbuch

Carvalhal AV, Moreira JL, Carrondo MJ
Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression
J Biotechnol, 92:47-59 More info

Fonseca LL, Alves PM, Carrondo MJ, Santos H
Effect of ethanol on the metabolism of primary astrocytes studied by [13C]- and [31P]-NMR spectroscopy
J Neurosci Res, 66:803-11 More info

Merten OW, Cruz PE, Rochette C, Geny-Fiamma C, Bouquet C, Goncalves D, Danos O, Carrondo MJ
Comparison of different bioreactor systems for the production of high titer retroviral vectors
Biotechnol Prog, 17:326-35 More info

Morais VA, Serpa J, Palma AS, Costa T, Maranga L, Costa J, Wu N, Ataai MM
Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system
Biochem J, 353:719-25 More info


Alves PM, Fonseca LL, Peixoto CC, Almeida AC, Carrondo MJ, Santos H
NMR studies on energy metabolism of immobilized primary neurons and astrocytes during hypoxia, ischemia and hypoglycemia
NMR Biomed, 13:438-48 More info

Alves PM, Nunes R, Zhang C, Maycock CD, Sonnewald U, Carrondo MJ, Santos H
Metabolism of 3-[13C]malate in primary cultures of mouse astrocytes
Dev Neurosci, 22:456-62 More info

Araujo C, Marques S, Carrondo MJ, Gonçalves L
In vitro response of brown bullhead catfish (BB) and rainbow trout (RTG-2) cell lines to benzo[a]pyrene
Sci Total Environ, 247:127-35 More info

Carvalhal AV, Moreira JL, Cruz H, Mueller P, Hauser H, Carrondo MJ
Manipulation of culture conditions for BHK cell growth inhibition by IRF-1 activation
Cytechnology, 32:135-45 More info

Cruz HJ, Dias EM, Peixoto CM, Moreira JL, Carrondo MJ
Product quality of a recombinant fusion protein expressed in immobilised baby hamster cells grown in protein-free medium
Biotechnol Lett, 22:677-82 More info

Cruz HJ, Freitas CM, Alves PM, Moreira JL, Carrondo MJ
Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells
Enzyme Microb Technol, 27:45-52 More info

Cruz HJ, Moreira JL, Carrondo MJ
Metabolically optimised BHK cell fed-batch cultures
J Biotechnol, 80:109-18 More info

Cruz HJ, Peixoto CM, Nimtz M, Alves PM, Dias EM, Moreira JL, Carrondo MJ
Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells
Biotechnol Bioeng, 69:129-39 More info

Cruz PE, Almeida JS, Murphy PN, Moreira JL, Carrondo MJ
Modeling retrovirus production for gene therapy. 1. Determination of optimal bioreaction mode and harvest strategy
Biotechnol Prog, 16:213-21 More info

Cruz PE, Goncalves D, Almeida J, Moreira JL, Carrondo MJ
Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing
Biotechnol Prog, 16:350-57 More info

Cruz PE, Peixoto CC, Devos K, Moreira JL, Saman E, Carrondo MJ
Characterization and downstream processing of HIV-1 core and virus-like-particles produced in serum free medium
Enzyme Microb Technol, 26:61-70 More info


Cruz HJ, JL Moreira, MJ Carrondo
Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells
Biotechnol Bioeng, 66:104-13 More info

Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJT
Proteolytic Activity in Infected and non-Infected Insect Cells: Degradation of HIV-1 Pr55gag Particles
Biotechnology and Bioengineering, 65:133-143.  More info

Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJT
Proteolytic Activity in Infected and non-Infected Insect Cells: Degradation of HIV-1 Pr55gag Particles
Biotechnology and Bioengineering, 65:133-143. 


Alves PM, Flögel U, Brand A, Leibfritz D, Carrondo MJT, Santos H, Sonnewald, U
Immobilization of Primary Astrocytes and Neurons for NMR Monitoring of Biological Processes in Vivo
Developmental Neuroscience, 18:478‑483.  More info

Alves PM, Moreira JL, Rodrigues JM, Aunins JG, Carrondo, MJT
Two Dimensional versus Three-Dimensional Cultures Systems: Effects on Growth and Productivity of BHK Cells
Biotechnology and Bioengineering, 52:429-432. 


Alves PM, Mckenna MC, Sonnewald U
Lactate Metabolism in Mouse Brain Astrocytes Studied by [13C] NMR Spectroscopy
NeuroReport, 6, 2201-2204.  More info

Moreira JL, Alves PM, Aunins JG, Carrondo MJT
Hydrodynamic effects on BHK cells grown as suspended natural aggregates
Biotechnology and Bioengineering, 46:351-360.  More info

Moreira JL, Alves PM, Feliciano AS, Carrondo MJT
Serum Free and Containing Medium for Growth of Suspended BHK Natural Aggregates in Stirred Vessels
Enzyme Microbiology Technology, 17:437-444.  More info


Moreira JL, Alves PM, Aunins JG, Carrondo MJT
Changes in Animal Cell Natural Aggregates in Suspended Batch Cultures
Applied Microbiology and Biotechnology, 41:203-209.  More info

Moreira JL, Alves PM, Rodrigues JM, Cruz PE, Aunins JG, Carrondo MJT
Studies of Baby Hamster Kidney Natural Cell Aggregation in Suspended Batch Cultures
Annals of the New York Academy of Sciences, 745:122-133.  More info

Racher AJ, Moreira JL, Alves PM, Wirth M, Weidle UH, Hauser H., Carrondo MJT, Griffiths JB
Expression of Recombinant Antibody and Secreted Alkaline Phosphatase in Recombinant Animal Cells. Influence of Cell Line and Culture System upon Production Kinetics
Applied Microbiology and Biotechnology, 40:851-856.   More info