Structural Biology for Drug Discovery
The Structural Biology for Drug Discovery Infrastructure Unit has strong competences and an extensive track record in 3D protein structure determination by X-ray crystallography, along with expertise in Limited Proteolysis for protein construct design, and Differential Fluorimetry Assays (Thermofluor) for protein stability studies.
Diffraction data measurements can be done in-house or at Synchrotron Radiation facilities in Europe. If small target proteins fail to crystallize, structural NMR can be used, with access to a wide range of spectrometers located at CERMAX in ITQB. NMR spectroscopy can also be used for drug screening and hit optimization, mapping of protein-ligand interactions during drug discovery process, structure-activity relationships and studies of protein-protein complexes.
Finally, extensive competences and experience in molecular modeling of protein structures, protein-ligand and protein-protein interactions is also available, with access to in-house state-of-the-art computing facilities.