On 20 January, associate scientist Giacomo Domenici (Advanced Cell Models lab) gave a talk entitled “Establishment of PDX-Derived Ewing’s Sarcoma 3d Cell Cultures Employing Spheroid Encapsulation” in an online seminar series on Sarcoma Biology organized by the European Network for Sarcoma (EuSARC).
This work consisted in the development of a 3D cell model based on Ewing’s Sarcoma patient-derived xenograft (PDX) cell spheroids encapsulated into alginate hydrogels and maintained in agitation-based culture systems. This methodology yielded cultures with high cell viability and preserved intrinsic characteristics of Ewing’s Sarcoma tumour cells for, at least, one month, overcoming some limitations offered by other PDX-derived models cultured on 2D surfaces.
Ewing’s Sarcoma is the second most frequent bone tumour in children and young adults, with very aggressive behaviour and significant disease recurrence. This novel 3D cell model offers new opportunities to better study the pathobiology of the disease and to develop novel anticancer drugs.