Research Fellowship (MSc) – Cell Line Dev. & Molecular Virology

Instituto de Biologia Experimental e Tecnológica (iBET), Oeiras, Portugal, has an open position for a research fellowship in the field of Biotechnology available within the project “NAAVigate-Novel engineering strategies to unblock AAV vector cellular trafficking: enhancing transduction efficiencies Ref. 779/780, financed by iBET funds through iBETxplore.

Education:

• Minimum of Master’s degree with a grade of 18 the or above.

Professional experience:

• Minimum 1-year laboratory experience in molecular biology techniques.
• Minimum 1-year laboratory experience in mammalian cell culture.
• Laboratory experience in constructing expression plasmids for mammalian cells.
• Laboratory experience in mammalian cell transfection.
• Laboratory experience in viral vector production (AAV, LV) for gene therapy and viral titration assays.
• Experience in flow cytometry, fluorescence microscopy, and qPCR techniques.
• Proficient in English.
• Motivated for research in the field of Animal Cell Biotechnology.
• Strong organizational, communication, and teamwork skills.
• Immediate availability.

The fellow will provide technical and scientific support to the mentioned project, particularly in plasmid construction, production and titration of adeno-associated viral vectors, and transduction assays by cytometry, microscopy and PCR assays (qPCR and ddPCR).

The fellowship will have a duration of 12 months, eventually renewed until the end of project, without prejudice of the maximum limits foreseen by law. The expected starting date is 1 of July of 2024.