Bolsa de Investigação para Mestre no âmbito do projeto “SILVERBRAIN"
ref: Ref. SIVERBRAIN_Oct_2020
area: Ciências da Saúde, Bioquímica, Biologia, Farmácia ou áreas afins.
available vacancies: 1
Encontra-se aberto concurso para a atribuição de uma Bolsa de Investigação para Mestre para atividade de investigação no âmbito do projeto “SILVERBRAIN “, Ref. PTDC/OCE-ETA/30240/2017), “From sea to brain: Green neuroprotective extracts for nanoencapsulation and functional food production”
Plano de trabalho:
Algae extracts will be screened for their bioactivities in order to discover the active compounds resulting from seaweeds. Bioactivity screening will underlie neurodegenerative diseases and inflammation and will be evaluated using a High-Throughput Screening (HTS) – based on a SMART (Simple modular architecture research tool) yeast screening platform. Those with potential anti-inflammatory properties will be screened using the yeast Ca2+/calcineurin/Crz1 reporter assay, where Crz1 is the yeast homologue of human NFAT (Nuclear factor of activated T-cells). This anti-inflammatory potential will be validated in mammalian cell models. The last goal will be to evaluated the extracts BBB permeability and transport on confluent monolayers of brain microvascular endothelial cells, assessing its ability to permeate the BBB and determine the apparent permeability and extent of absorption.