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Biologics



biologics@ibet.pt

Explore R&D Services

Explore R&D Services

iBET’s Bioproduction Unit offer research and development (R&D), process development and manufacturing services

Protein production services include the production and purification of recombinant proteins, antibodies, enzymes and protein complexes

FULL RANGE SERVICE: FROM GENE TO PROTEIN

Our services: molecular cloning, feasibility studies, manufacturing and technology transfer.

Molecular Biology

  • Reverse translation of the protein of interest
  • Host specific codon optimization
  • Vector construction
  • Special features – e.g., inclusion of tags for purification, insertion of protease signal for tag removal, signal peptides for secretion

Protein Expression

  • Expression systems:
    • Bacteria (E. coli);
    • Yeast;
    • Baculovirus-insect cell system (BEVS, Sf9, Sf21 and Hi5);
    • Mammalian cells (HEK293, PerC6, CAP, Caco2, MRC-5, Sp2/0, BAE, Vero, MDCK, DK, CHO, A549)
  • Stable or transient systems
  • Small scale to large scale (50L working volume)

Protein Purification and Isolation

  • Purification of secreted and intracellular targets (tagged or untagged)
  • Purification of proteins from inclusion bodies, including refolding
  • Membrane technology: ÄKTA Crossflow – scalable, ultrafiltration systems
  • Chromatography: ÄKTA Platform Technology (Explorer, Avant, Pure and Pilot)

Quality Control

  • Broad range of assays for concentration (A280, BCA and HPLC) and purity (MS, HPLC, gel electrophoresis, Western Blot, Endotoxin level and Sterility tests) – ELISA and product specific functional assays
  • Infectious and total viral particle quantification (plaque assay, qPCR)
  • Stability testing

Protein Characterization

  • Molecular mass determination
  • Peptide mass fingerprinting
  • Post-translational modifications profiling (e.g., glycosylation and phosphorylation)
  • Determination of Isoelectric point
  • Immunoassays
  • Aggregation and refolding (SEC and RP chromatography)
  • Functional characterization (potency determination)
  • Biophysical characterization (purity, solubility, viscosity, hydrophobicity and thermal, structural and chemical stability)

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