back to R&D Services
Contact us

Biologics



biologics@ibet.pt

Explore R&D Services

Explore R&D Services

At iBET we offer research and development (R&D), process development and manufacturing services. Our services range from feasibility studies in small and large scale to technology transfer

Virus production services include the production and purification of replicative virus and non-replicative viral particles. We have experience with retrovirus, lentivirus, adenovirus, adeno-associated virus, rhabdovirus, baculovirus and virus-like particles

Virus Production

Virus Purification

  • Purification of secreted and intracellular viral particles
  • Ultracentrifugation
  • Membrane technology: ÄKTA Crossflow – scalable, ultrafiltration systems
  • Chromatography: ÄKTA Platform Technology (Explorer, Avant, Pure and Pilot)

Quality Testing

  • Concentration determination (A280, BCA, and HPLC, Titration Assays)
  • Purity determination (MS, HPLC, Gel Electrophoresis, Host DNA and Host Protein Quantification, Endotoxin level and Sterility tests)
  • Infectious and total viral particle quantification (plaque assay, qPCR)
  • Stability testing

Virus Characterization

  • Electron microscopy
  • Aggregation (NTA, qNANO/TRPS)
  • Functional characterization (potency determination)
  • Formulation (lyophilisation)
  • Biophysical characterization (purity, solubility, viscosity)

Associated Services

At iBET, we develop recombinant mammalian and insect cell lines for stable and transient viral expression. Advanced molecular biotechnology and synthetic biology tools, such as recombinase mediated cassette exchange (RMCE), methabolic engineering and CRISPR-CAS9 tools are used for cell line development.

We also have expertise in on-line monitoring virus-production through in-house developed system-biology methods. Furthermore, our highly-skilled professionals employ a variety of analytical assays to characterize producer cells and virus quality.

You may also be interested in

Filter your search by

Definições de Cookies

O iBET pode utilizar cookies para memorizar os seus dados de início de sessão, recolher estatísticas para otimizar a funcionalidade do site e para realizar ações de marketing com base nos seus interesses.

Permitem personalizar as ofertas comerciais que lhe são apresentadas, direcionando-as para os seus interesses. Podem ser cookies próprios ou de terceiros. Alertamos que, mesmo não aceitando estes cookies, irá receber ofertas comerciais, mas sem corresponderem às suas preferências.
Oferecem uma experiência mais personalizada e completa, permitem guardar preferências, mostrar-lhe conteúdos relevantes para o seu gosto e enviar-lhe os alertas que tenha solicitado.
Permitem-lhe estar em contacto com a sua rede social, partilhar conteúdos, enviar e divulgar comentários.