Flow Cytometry Unit
Empowering Cell and Gene Therapy through advanced single-cell analysis and cell sorting
Flow Cytometry at iBET
Established in late 2025, iBET’s Flow Cytometry Unit strengthens the institute’s bioanalytical platform by providing state-of-the-art cell analysis and high-speed sorting capabilities to support internal R&D activities in advanced Cell and Gene Therapy products.
The unit offers specialized single-cell, multiparametric phenotyping capabilities, alongside routine and custom assays using conventional and spectral flow cytometry.
The Flow Cytometry Unit is committed to delivering high‑quality, high‑resolution data that accelerates innovation in cell characterization, immune profiling, and the development of advanced therapeutic products. It also enables high-speed single-cell selection and isolation of cell products to support downstream functional assays, such as single-cell RNA sequencing and clonal cell line generation.
The unit supports iBET’s internal R&D projects, including those in collaboration with various academic and industrial partners.
Flow Cytometry Assays
Flow Cytometry can be used to perform a range of cell-based assays that assess identity, purity, viability, phenotype, and functional activity at the single-cell level.
Biological Products
Projects and R&D services provided by the Flow Cytometry Unit cover the characterization of a wide range of biological products:
Equipment & Facilities
The Flow Cytometry Unit is equipped with state-of-the-art cytometers, including cell analyzers (Spectral Sony ID7000, BD FACSCelesta) and a high-speed cell sorter (Sony MA900).
Located within the iBET Biofarma building (state-of-the-art facilities inaugurated in 2023), this unit operates in close complementarity with the Mass Spectrometry Unit and the GMP-certified Analytical Services Unit (ASU), reinforcing iBET’s integrated bioanalytical capabilities.
The following equipment is available in this unit:
Conventional analyzer, 4-laser configuration (6V-3B-4YG-3R) consisting of a violet (405 nm), blue (488 nm), yellow-green (561 nm) and a red (640 nm) laser. It has 2 scatter and 16 fluorescence detectors. It is equipped with a high-throughput sampler for 96-well and 384-well plates.
Full-spectrum analyzer, 4 laser configuration (35V-32B-26YG-19R), a violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (637 nm) laser. It has 32-channel PMTs, 2 scatter channels and 112 fluorescent detectors. It is equipped with a robust auto-sampler, perfect for running samples from multiple 96-well, 384-well plates or 5ml tubes from a 24-tube rack.
Conventional cell sorter, 4 laser configuration a violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (637 nm) laser. It has 2 scatter and 12 fluorescence detectors. It can perform 2-way or 4-way tube sorts and 96- and 384-well plate or PCR plate sorting. It can be setup to sort cells or particles of variable sizes using 70 μm, 100 μm or 130 μm microfluidics sorting chip and process up to 12,000 events per second using a 70 μm chip.
Olivia Rodrigues
Head of Flow Cytometry Unit
My current research focuses on bridging science and technology by advancing next-generation flow cytometry platforms at iBET. The goal is to develop advanced flow cytometry analytics and single-cell sorting tools that accelerate bioprocesses and the manufacturing of next-generation cell therapies.